miRNA: 3'- uCCCG-UAGU-------UGUAGCGGau--GCAGCUg -5'
miRNA: 3'- uCCCg-UAGUuGUAGCGGAUG-------CAGCUG- -5'
miRNA: 3'- uccCGUAGUUGUa-----GCGGAUG-CAGCUG- -5'
miRNA: 3'- -UCCCGua--GUUGuaGCGGAugCAGCUG- -5'
miRNA: 3'- -UCCCGUAGUUGUAGCGGAUGCAGCUG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7245 | 5' | -53.3 | NC_001900.1 | + | 36586 | 0.71 | 0.473315 |
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Target: 5'- cGGGCuGUCGgugccguGCAUCGCCgcgcCGUCGAa -3' miRNA: 3'- uCCCG-UAGU-------UGUAGCGGau--GCAGCUg -5' |
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| 7245 | 5' | -53.3 | NC_001900.1 | + | 5299 | 0.72 | 0.413339 |
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Target: 5'- cGGGaaGUCAuCAUCGCCUACccagaugGUCGGCg -3' miRNA: 3'- uCCCg-UAGUuGUAGCGGAUG-------CAGCUG- -5' |
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| 7245 | 5' | -53.3 | NC_001900.1 | + | 23574 | 0.73 | 0.395343 |
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Target: 5'- --cGCGUCAACGacaacgCGCCUACgGUCGGCa -3' miRNA: 3'- uccCGUAGUUGUa-----GCGGAUG-CAGCUG- -5' |
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| 7245 | 5' | -53.3 | NC_001900.1 | + | 14077 | 0.78 | 0.202674 |
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Target: 5'- gAGGGCugggCAGC--CGCCUugGUCGACc -3' miRNA: 3'- -UCCCGua--GUUGuaGCGGAugCAGCUG- -5' |
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| 7245 | 5' | -53.3 | NC_001900.1 | + | 2174 | 1.11 | 0.00102 |
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Target: 5'- aAGGGCAUCAACAUCGCCUACGUCGACa -3' miRNA: 3'- -UCCCGUAGUUGUAGCGGAUGCAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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