Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7251 | 3' | -54.7 | NC_001900.1 | + | 9303 | 0.66 | 0.699013 |
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Target: 5'- gGCGU-CACgGUGCCCGUccagaugcagucACuGCUGGCu -3' miRNA: 3'- gUGCGuGUGaCACGGGUA------------UG-CGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 48842 | 0.66 | 0.687904 |
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Target: 5'- -gUGUGCGCUgGUGUgUAUGCGCUGGu -3' miRNA: 3'- guGCGUGUGA-CACGgGUAUGCGACUg -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 17817 | 0.68 | 0.586758 |
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Target: 5'- gGCaGCACaaccuGCUGgcUGCCCGguucguCGCUGACg -3' miRNA: 3'- gUG-CGUG-----UGAC--ACGGGUau----GCGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 44777 | 0.68 | 0.553398 |
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Target: 5'- cCACGCu--CUGgggaaccGCCCGcUugGCUGACg -3' miRNA: 3'- -GUGCGuguGACa------CGGGU-AugCGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 8514 | 0.69 | 0.499202 |
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Target: 5'- gCACGCGcCACUGcaugcgugagGCCCAgaagGCUGGCa -3' miRNA: 3'- -GUGCGU-GUGACa---------CGGGUaug-CGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 4725 | 0.7 | 0.431582 |
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Target: 5'- cCACGCGCAguggguccacgacaUGUGCCgCGcucGCGCUGGCa -3' miRNA: 3'- -GUGCGUGUg-------------ACACGG-GUa--UGCGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 17557 | 0.71 | 0.37141 |
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Target: 5'- uCAU-CGCGCUGaUGCCC--GCGCUGACu -3' miRNA: 3'- -GUGcGUGUGAC-ACGGGuaUGCGACUG- -5' |
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| 7251 | 3' | -54.7 | NC_001900.1 | + | 197 | 1.09 | 0.000866 |
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Target: 5'- aCACGCACACUGUGCCCAUACGCUGACc -3' miRNA: 3'- -GUGCGUGUGACACGGGUAUGCGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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