miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7252 3' -60.9 NC_001900.1 + 6625 0.66 0.422037
Target:  5'- uUCACGGCACGgGccaGCC-CGAuccccucGGGCc -3'
miRNA:   3'- gAGUGCCGUGCgCa--CGGaGCUc------CCCG- -5'
7252 3' -60.9 NC_001900.1 + 40545 0.66 0.398611
Target:  5'- uUCugGGCcucacgcaugcagugGCGCGUGCCgacugaccuacCGAGaGGGa -3'
miRNA:   3'- gAGugCCG---------------UGCGCACGGa----------GCUC-CCCg -5'
7252 3' -60.9 NC_001900.1 + 43370 0.67 0.360919
Target:  5'- aUC-CGGCGCGUGaccGUUUCGAGGucgcGGCg -3'
miRNA:   3'- gAGuGCCGUGCGCa--CGGAGCUCC----CCG- -5'
7252 3' -60.9 NC_001900.1 + 25421 0.69 0.264615
Target:  5'- -gCGCGGaguCGUGUGCC-CGAGGcGCa -3'
miRNA:   3'- gaGUGCCgu-GCGCACGGaGCUCCcCG- -5'
7252 3' -60.9 NC_001900.1 + 48526 0.69 0.264615
Target:  5'- gUCGCGGCAUGUuccagGCCgCGAGGuuGGCc -3'
miRNA:   3'- gAGUGCCGUGCGca---CGGaGCUCC--CCG- -5'
7252 3' -60.9 NC_001900.1 + 13982 0.69 0.251796
Target:  5'- -aCGuCGGCACGaCGUGCUgagcaggacaaCGGGGGGUu -3'
miRNA:   3'- gaGU-GCCGUGC-GCACGGa----------GCUCCCCG- -5'
7252 3' -60.9 NC_001900.1 + 48967 0.7 0.233531
Target:  5'- -gCugGGCAuCGUGUGCCccUCGAGGcacGCg -3'
miRNA:   3'- gaGugCCGU-GCGCACGG--AGCUCCc--CG- -5'
7252 3' -60.9 NC_001900.1 + 3798 0.7 0.233531
Target:  5'- uUCGCGGUcgGCGUGaCCUCGAcGGcGGUc -3'
miRNA:   3'- gAGUGCCGugCGCAC-GGAGCU-CC-CCG- -5'
7252 3' -60.9 NC_001900.1 + 42965 0.74 0.127431
Target:  5'- aUCugGGCcagcacguuccgaGCGCGUGCgaugaaCUCGGGGGaGCg -3'
miRNA:   3'- gAGugCCG-------------UGCGCACG------GAGCUCCC-CG- -5'
7252 3' -60.9 NC_001900.1 + 81 1.11 0.000181
Target:  5'- gCUCACGGCACGCGUGCCUCGAGGGGCa -3'
miRNA:   3'- -GAGUGCCGUGCGCACGGAGCUCCCCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.