Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7255 | 3' | -56.5 | NC_001900.1 | + | 39216 | 0.66 | 0.592681 |
Target: 5'- uACCUGCGUaagcccCCGGCCGuCCaggucaUCGgGGa -3' miRNA: 3'- uUGGAUGCAa-----GGCCGGUuGG------AGCgCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 31650 | 0.66 | 0.647512 |
Target: 5'- uGACgaACGcUUCaccgaaGGUCcGCCUCGCGGu -3' miRNA: 3'- -UUGgaUGC-AAGg-----CCGGuUGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 45677 | 0.66 | 0.644219 |
Target: 5'- cGCUcACGg-CCGGCCGGCCUcaccacaccuuucgCGUGGg -3' miRNA: 3'- uUGGaUGCaaGGCCGGUUGGA--------------GCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 16935 | 0.66 | 0.636534 |
Target: 5'- uGGCCUACuggCUGGCCuGCCgUCGCu- -3' miRNA: 3'- -UUGGAUGcaaGGCCGGuUGG-AGCGcc -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 47616 | 0.66 | 0.625553 |
Target: 5'- gAACCUucggaGCGUUCuuacgCGGU--GCCUUGCGGa -3' miRNA: 3'- -UUGGA-----UGCAAG-----GCCGguUGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 1278 | 0.66 | 0.646415 |
Target: 5'- uGCCUGCGUUgCCGGugccuaaaccgccCCGGCCgcuuccgGCGGc -3' miRNA: 3'- uUGGAUGCAA-GGCC-------------GGUUGGag-----CGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 46732 | 0.67 | 0.528045 |
Target: 5'- cGCCUacaugcaagagACGUUCgggguCGGCCGACgagacaUCGCGGa -3' miRNA: 3'- uUGGA-----------UGCAAG-----GCCGGUUGg-----AGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 25237 | 0.67 | 0.549351 |
Target: 5'- cGCCU-CGUacCCGGCCcgAGCCUCuGCGa -3' miRNA: 3'- uUGGAuGCAa-GGCCGG--UUGGAG-CGCc -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 43312 | 0.67 | 0.550424 |
Target: 5'- gGACCgagACuGggCUGGCCcggaccacgaccuccACCUCGCGGa -3' miRNA: 3'- -UUGGa--UG-CaaGGCCGGu--------------UGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 9698 | 0.67 | 0.570917 |
Target: 5'- uGCCUugG--CCGGCCGACCgaucCGaGGa -3' miRNA: 3'- uUGGAugCaaGGCCGGUUGGa---GCgCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 16518 | 0.68 | 0.507053 |
Target: 5'- cAGCCUuc-UUCgCGGCCAcuACCaUCGCGGc -3' miRNA: 3'- -UUGGAugcAAG-GCCGGU--UGG-AGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 47360 | 0.68 | 0.486424 |
Target: 5'- cGACC-GCGUUCUGuGCCAagguguGCUaCGCGGg -3' miRNA: 3'- -UUGGaUGCAAGGC-CGGU------UGGaGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 41216 | 0.68 | 0.47626 |
Target: 5'- cACCUGCugGUgcagCgGGUUGGCCUUGCGGg -3' miRNA: 3'- uUGGAUG--CAa---GgCCGGUUGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 18810 | 0.68 | 0.517506 |
Target: 5'- uGAUgaACGcaCCGGUCGACUUCGCGa -3' miRNA: 3'- -UUGgaUGCaaGGCCGGUUGGAGCGCc -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 7218 | 0.69 | 0.456256 |
Target: 5'- uGGCCUucaucACGaUCCGGCCGauggcgACCUCGUa- -3' miRNA: 3'- -UUGGA-----UGCaAGGCCGGU------UGGAGCGcc -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 47842 | 0.7 | 0.390052 |
Target: 5'- gGACUcGCGUgCCGugacGCgAACCUCGCGGu -3' miRNA: 3'- -UUGGaUGCAaGGC----CGgUUGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 25634 | 0.7 | 0.408325 |
Target: 5'- cAUCUuCG-UCCGGUCAGCCaugugcUCGCGGa -3' miRNA: 3'- uUGGAuGCaAGGCCGGUUGG------AGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 41077 | 0.74 | 0.21455 |
Target: 5'- aGACgUUGCGggCCaGGCCGgugACCUCGCGGc -3' miRNA: 3'- -UUG-GAUGCaaGG-CCGGU---UGGAGCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 16616 | 0.75 | 0.192907 |
Target: 5'- cGGCCgACGUUCCGgucaacGCCGACCUgaaCGCGGc -3' miRNA: 3'- -UUGGaUGCAAGGC------CGGUUGGA---GCGCC- -5' |
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7255 | 3' | -56.5 | NC_001900.1 | + | 519 | 1.07 | 0.000936 |
Target: 5'- cAACCUACGUUCCGGCCAACCUCGCGGc -3' miRNA: 3'- -UUGGAUGCAAGGCCGGUUGGAGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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