Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7256 | 3' | -60 | NC_001900.1 | + | 22224 | 0.66 | 0.462606 |
Target: 5'- uGUCUGGgauacgcacggguUCuGGGUCgGGAGCuCCUUCu -3' miRNA: 3'- gCAGACC-------------AG-CUCGGgCCUCGcGGAAG- -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 49025 | 0.66 | 0.457745 |
Target: 5'- uCGUCgcaGGUCacgGGGgguagggggguucccCCCaGGGGCGCCUUCc -3' miRNA: 3'- -GCAGa--CCAG---CUC---------------GGG-CCUCGCGGAAG- -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 2536 | 0.67 | 0.398092 |
Target: 5'- aCGUC-GGUgGcAGCCCGGAcGCaGCCg-- -3' miRNA: 3'- -GCAGaCCAgC-UCGGGCCU-CG-CGGaag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 18750 | 0.69 | 0.286263 |
Target: 5'- -aUCUGGUCGAgcugcGCCUGcAGCGCCg-- -3' miRNA: 3'- gcAGACCAGCU-----CGGGCcUCGCGGaag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 33705 | 0.69 | 0.279299 |
Target: 5'- aCGUCUGGUagaGcGCCUGGAaCGCCa-- -3' miRNA: 3'- -GCAGACCAg--CuCGGGCCUcGCGGaag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 13462 | 0.69 | 0.27247 |
Target: 5'- gCGUCUGGUgCGuaaCCCGGAGgGCUa-- -3' miRNA: 3'- -GCAGACCA-GCuc-GGGCCUCgCGGaag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 23164 | 0.7 | 0.246494 |
Target: 5'- aCGUCUGcUgGAGCCCGGAG-GCUa-- -3' miRNA: 3'- -GCAGACcAgCUCGGGCCUCgCGGaag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 3999 | 0.71 | 0.228385 |
Target: 5'- cCGUCucggUGGUCGAGCCaggcaaGGAcCGCCUg- -3' miRNA: 3'- -GCAG----ACCAGCUCGGg-----CCUcGCGGAag -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 23216 | 0.76 | 0.094139 |
Target: 5'- gGUCUGGUCcucGCCCGGGcuGCGCCcgUCa -3' miRNA: 3'- gCAGACCAGcu-CGGGCCU--CGCGGa-AG- -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 11618 | 0.78 | 0.061798 |
Target: 5'- gGUCgGGUCGccGgCCGGAGUGCCUUCg -3' miRNA: 3'- gCAGaCCAGCu-CgGGCCUCGCGGAAG- -5' |
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7256 | 3' | -60 | NC_001900.1 | + | 630 | 1.09 | 0.000313 |
Target: 5'- cCGUCUGGUCGAGCCCGGAGCGCCUUCu -3' miRNA: 3'- -GCAGACCAGCUCGGGCCUCGCGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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