Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7258 | 5' | -56.4 | NC_001900.1 | + | 21797 | 0.66 | 0.571625 |
Target: 5'- cGGCGCUaugGGACAAGCgg--GGAUUCu -3' miRNA: 3'- uUCGUGGa--CCUGUUCGaggaCCUGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 20909 | 0.66 | 0.556074 |
Target: 5'- gGGGCACCgagaccgccuucGGC-GGCUCCUGG-CUCa -3' miRNA: 3'- -UUCGUGGac----------CUGuUCGAGGACCuGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 13923 | 0.66 | 0.549446 |
Target: 5'- cGAGCACCggcUGGuacCGGGCUCCgguaucgagGaGGCUCa -3' miRNA: 3'- -UUCGUGG---ACCu--GUUCGAGGa--------C-CUGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 9376 | 0.66 | 0.549446 |
Target: 5'- -uGCAUCUGGACGGGCaCCgugacgccgaUGGcCUCu -3' miRNA: 3'- uuCGUGGACCUGUUCGaGG----------ACCuGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 154 | 0.66 | 0.549446 |
Target: 5'- uGGCuCCUGGugGcucacAGCgCCUGGGCg- -3' miRNA: 3'- uUCGuGGACCugU-----UCGaGGACCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 43989 | 0.66 | 0.527541 |
Target: 5'- cAGCACCgGGACGAGCUacaCCuacgUGGAgCUg -3' miRNA: 3'- uUCGUGGaCCUGUUCGA---GG----ACCU-GAg -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 21048 | 0.66 | 0.527541 |
Target: 5'- uGGUuCCUGGGCAccAGC-CCgaGGGCUCc -3' miRNA: 3'- uUCGuGGACCUGU--UCGaGGa-CCUGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 19963 | 0.67 | 0.51671 |
Target: 5'- -cGCAUCgGGAUgcGCUCCgGGACa- -3' miRNA: 3'- uuCGUGGaCCUGuuCGAGGaCCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 30806 | 0.67 | 0.50597 |
Target: 5'- gAAGCACC-GGuCGAGCaCCUGGAg-- -3' miRNA: 3'- -UUCGUGGaCCuGUUCGaGGACCUgag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 1591 | 0.67 | 0.464033 |
Target: 5'- -uGCGCCUGGcuCAGGaUCUUGGACa- -3' miRNA: 3'- uuCGUGGACCu-GUUCgAGGACCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 12287 | 0.68 | 0.453833 |
Target: 5'- cGGCACCuucUGGGCGAaCUgCUGGACg- -3' miRNA: 3'- uUCGUGG---ACCUGUUcGAgGACCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 36492 | 0.68 | 0.453833 |
Target: 5'- gAGGUACCa-GACccacAGCUCCUGGACg- -3' miRNA: 3'- -UUCGUGGacCUGu---UCGAGGACCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 28698 | 0.68 | 0.433805 |
Target: 5'- -uGUACCaGGGC-AGCUCCUGGcCgUCg -3' miRNA: 3'- uuCGUGGaCCUGuUCGAGGACCuG-AG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 45857 | 0.68 | 0.423985 |
Target: 5'- -cGCACCgugaGGGCAAGC-CgUGGuACUCc -3' miRNA: 3'- uuCGUGGa---CCUGUUCGaGgACC-UGAG- -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 16840 | 0.82 | 0.044867 |
Target: 5'- cAGCACCUGGGCAAGUcguUCCUGGAUc- -3' miRNA: 3'- uUCGUGGACCUGUUCG---AGGACCUGag -5' |
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7258 | 5' | -56.4 | NC_001900.1 | + | 1559 | 1.08 | 0.000569 |
Target: 5'- gAAGCACCUGGACAAGCUCCUGGACUCg -3' miRNA: 3'- -UUCGUGGACCUGUUCGAGGACCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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