Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7259 | 5' | -56.6 | NC_001900.1 | + | 37040 | 0.66 | 0.626967 |
Target: 5'- cCGGCaaGGCaUCAAGCCCcacaccuGGGGCuacgGAg -3' miRNA: 3'- -GCUG--CCGcAGUUCGGGu------UCCCGua--CU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 46006 | 0.66 | 0.626967 |
Target: 5'- aGGCGGUGUCu--UCCAGGGGUucAUGc -3' miRNA: 3'- gCUGCCGCAGuucGGGUUCCCG--UACu -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 33727 | 0.66 | 0.625865 |
Target: 5'- -cACGGCcaacGUCGAcccggacGCCUacGAGGGCGUGGa -3' miRNA: 3'- gcUGCCG----CAGUU-------CGGG--UUCCCGUACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 11585 | 0.66 | 0.572121 |
Target: 5'- -uGCGGUGUCGGacauguguuuucGCCUccuGGGCAUGGu -3' miRNA: 3'- gcUGCCGCAGUU------------CGGGuu-CCCGUACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 33553 | 0.67 | 0.561265 |
Target: 5'- uGACGGUGUCGAugaagaguGCgCCAAcGGcGCAUGc -3' miRNA: 3'- gCUGCCGCAGUU--------CG-GGUU-CC-CGUACu -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 9555 | 0.67 | 0.550467 |
Target: 5'- gGACGuaaGCGUCGguguAGCCCAgcGGGGCcucGAu -3' miRNA: 3'- gCUGC---CGCAGU----UCGGGU--UCCCGua-CU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 2573 | 0.67 | 0.550467 |
Target: 5'- uGAgGGCGU--AGCCCGAcGGCggGAa -3' miRNA: 3'- gCUgCCGCAguUCGGGUUcCCGuaCU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 21134 | 0.67 | 0.539733 |
Target: 5'- aCGACGuGCG--GAGCCCucGGGC-UGGu -3' miRNA: 3'- -GCUGC-CGCagUUCGGGuuCCCGuACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 6721 | 0.67 | 0.529071 |
Target: 5'- uCGGCaGGCagGcCAGGCCCGAGGGgAUcGGg -3' miRNA: 3'- -GCUG-CCG--CaGUUCGGGUUCCCgUA-CU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 25782 | 0.67 | 0.518488 |
Target: 5'- uCGuCGGCaacaccaagaccGUCAGGUCCAAGcGGCcgGUGAc -3' miRNA: 3'- -GCuGCCG------------CAGUUCGGGUUC-CCG--UACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 36271 | 0.68 | 0.497582 |
Target: 5'- aCGACGGCGUCGu-CCCGgagAGGGguUc- -3' miRNA: 3'- -GCUGCCGCAGUucGGGU---UCCCguAcu -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 15173 | 0.68 | 0.487271 |
Target: 5'- gGGCGGCGauucCGAGgUCAAGGGCuccUGGc -3' miRNA: 3'- gCUGCCGCa---GUUCgGGUUCCCGu--ACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 32317 | 0.69 | 0.447096 |
Target: 5'- cCGAUGGuCGUCAcgGGCaaCCAccGGGCGUGGc -3' miRNA: 3'- -GCUGCC-GCAGU--UCG--GGUu-CCCGUACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 28260 | 0.69 | 0.437341 |
Target: 5'- uCGACGGa-UCAGGUCCGggAGGGCuccGUGGa -3' miRNA: 3'- -GCUGCCgcAGUUCGGGU--UCCCG---UACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 35942 | 0.69 | 0.424845 |
Target: 5'- uCGGCGGCucguagagguacucGUCGcgcAGCCCGGGGugcucgaccaGCAUGAu -3' miRNA: 3'- -GCUGCCG--------------CAGU---UCGGGUUCC----------CGUACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 36887 | 0.69 | 0.418206 |
Target: 5'- uGACGGCaaccacGUCAAGCCCGAGcuGGUuccGAu -3' miRNA: 3'- gCUGCCG------CAGUUCGGGUUC--CCGua-CU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 15468 | 0.69 | 0.40883 |
Target: 5'- uGugGGCGUgGuGgCCAAGGcGCAUGu -3' miRNA: 3'- gCugCCGCAgUuCgGGUUCC-CGUACu -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 14532 | 0.7 | 0.361403 |
Target: 5'- aGGCGGUuucguGUCGGuGCCCGcgaagaacccggacGGGGCGUGGc -3' miRNA: 3'- gCUGCCG-----CAGUU-CGGGU--------------UCCCGUACU- -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 10430 | 0.72 | 0.284937 |
Target: 5'- uGGCGGCGUC-AGCCUucGcGGCGUa- -3' miRNA: 3'- gCUGCCGCAGuUCGGGuuC-CCGUAcu -5' |
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7259 | 5' | -56.6 | NC_001900.1 | + | 1744 | 0.72 | 0.26879 |
Target: 5'- uGuACGGCaGcaccaagcucuaccUCAAGCCCGAGGuGCGUGAc -3' miRNA: 3'- gC-UGCCG-C--------------AGUUCGGGUUCC-CGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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