Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7261 | 5' | -56 | NC_001900.1 | + | 33777 | 0.66 | 0.687723 |
Target: 5'- cGGCGUcuc-GGCCGAaccgugcGGCCACGg -3' miRNA: 3'- -CCGCAagcaCCGGCUcuua---CCGGUGC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 40308 | 0.66 | 0.676879 |
Target: 5'- cGGCaGUgagcCGcugcGGCCGuGGuUGGCCGCGu -3' miRNA: 3'- -CCG-CAa---GCa---CCGGCuCUuACCGGUGC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 23314 | 0.66 | 0.669263 |
Target: 5'- cGGCGgUCGgccccggcugGGCCGAGuucuauaccggcggcGGCUACGg -3' miRNA: 3'- -CCGCaAGCa---------CCGGCUCuua------------CCGGUGC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 16967 | 0.66 | 0.665993 |
Target: 5'- aGCGUUCGgcgcugGaGCCGGu-GUGGUCGCa -3' miRNA: 3'- cCGCAAGCa-----C-CGGCUcuUACCGGUGc -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 23844 | 0.67 | 0.611298 |
Target: 5'- cGGCGcUgG-GGCUGGGAA-GGCCAgGc -3' miRNA: 3'- -CCGCaAgCaCCGGCUCUUaCCGGUgC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 6396 | 0.67 | 0.589478 |
Target: 5'- aGCGgcCGUGGCCGGugc-GGCUGCGc -3' miRNA: 3'- cCGCaaGCACCGGCUcuuaCCGGUGC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 30784 | 0.68 | 0.546329 |
Target: 5'- cGGCcuUUCGgGGCCGGuGggUucGGCCACu -3' miRNA: 3'- -CCGc-AAGCaCCGGCU-CuuA--CCGGUGc -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 2265 | 0.68 | 0.545261 |
Target: 5'- ---cUUgGUGGCCGAGAggcgggugaucacGUGGCCAUc -3' miRNA: 3'- ccgcAAgCACCGGCUCU-------------UACCGGUGc -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 2216 | 0.69 | 0.493928 |
Target: 5'- aGUGgg-GUGGCUGGGAG-GGCCugGa -3' miRNA: 3'- cCGCaagCACCGGCUCUUaCCGGugC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 3544 | 0.7 | 0.406078 |
Target: 5'- cGGCGcUCGgGGCCGGuAAcGGCCugGu -3' miRNA: 3'- -CCGCaAGCaCCGGCUcUUaCCGGugC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 47589 | 0.7 | 0.406078 |
Target: 5'- aGCGacguUUCGUcGGCCGGGggUGGCgAa- -3' miRNA: 3'- cCGC----AAGCA-CCGGCUCuuACCGgUgc -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 10661 | 0.72 | 0.336748 |
Target: 5'- aGGCGgaaucacccccUUCGaUGGCCGGuuGUGGCUugGg -3' miRNA: 3'- -CCGC-----------AAGC-ACCGGCUcuUACCGGugC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 15363 | 0.73 | 0.283493 |
Target: 5'- aGGCGUUCG-GGCCGuAGuacaGGCCgaGCGa -3' miRNA: 3'- -CCGCAAGCaCCGGC-UCuua-CCGG--UGC- -5' |
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7261 | 5' | -56 | NC_001900.1 | + | 2165 | 1.1 | 0.000682 |
Target: 5'- gGGCGUUCGUGGCCGAGAAUGGCCACGu -3' miRNA: 3'- -CCGCAAGCACCGGCUCUUACCGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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