Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7262 | 5' | -53.5 | NC_001900.1 | + | 3997 | 0.66 | 0.781066 |
Target: 5'- cACCGuCUCgGUGGUCgAGCCagGCAAGg -3' miRNA: 3'- cUGGU-GAG-CGCCAGaUUGGa-CGUUCg -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 1356 | 0.66 | 0.781066 |
Target: 5'- aACgCGCUgGCGGUCgccGCCcGCGaccAGCa -3' miRNA: 3'- cUG-GUGAgCGCCAGau-UGGaCGU---UCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 18501 | 0.66 | 0.770779 |
Target: 5'- aGGCC-CUgGCGG-CUGAgCUGUcucAGGCg -3' miRNA: 3'- -CUGGuGAgCGCCaGAUUgGACG---UUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 6635 | 0.66 | 0.760341 |
Target: 5'- gGGCCAgcccgaucccCUCG-GGcCUGGCCUGCcuGCc -3' miRNA: 3'- -CUGGU----------GAGCgCCaGAUUGGACGuuCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 23074 | 0.66 | 0.749765 |
Target: 5'- cGCC-CUUGgGGUUgaugccgagGGCCUGCAgcAGCa -3' miRNA: 3'- cUGGuGAGCgCCAGa--------UUGGACGU--UCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 48655 | 0.66 | 0.749765 |
Target: 5'- cGGCCGCUaGUGGguugcgUCUGGCCUGCcacacguGCc -3' miRNA: 3'- -CUGGUGAgCGCC------AGAUUGGACGuu-----CG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 37463 | 0.67 | 0.728248 |
Target: 5'- aGCUGCUUGCGGUCaucgaacggGAUCUGCGuGUc -3' miRNA: 3'- cUGGUGAGCGCCAGa--------UUGGACGUuCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 25737 | 0.67 | 0.717332 |
Target: 5'- uGCCGCcucacgUCGCGGcgaUGAUCcGCGAGCa -3' miRNA: 3'- cUGGUG------AGCGCCag-AUUGGaCGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 1846 | 0.67 | 0.706327 |
Target: 5'- aGCCGggUGaCGGcCUcGGCCUGCGGGCg -3' miRNA: 3'- cUGGUgaGC-GCCaGA-UUGGACGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 3699 | 0.67 | 0.6841 |
Target: 5'- uGACCAaCUgCuCGGUCagcGGCUUGCGGGCg -3' miRNA: 3'- -CUGGU-GA-GcGCCAGa--UUGGACGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 4206 | 0.67 | 0.6841 |
Target: 5'- cGGCCGCuUCGcCGGUCggcagaugAACCaacUGCcAGCg -3' miRNA: 3'- -CUGGUG-AGC-GCCAGa-------UUGG---ACGuUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 12585 | 0.69 | 0.60526 |
Target: 5'- uGGCC-UUgGUGGUCUGACCgaUGUAGGUc -3' miRNA: 3'- -CUGGuGAgCGCCAGAUUGG--ACGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 15881 | 0.69 | 0.594011 |
Target: 5'- cGACCACggcuUCGCGGgccuUCUGGCCcaGCuucAGCa -3' miRNA: 3'- -CUGGUG----AGCGCC----AGAUUGGa-CGu--UCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 2589 | 0.71 | 0.484785 |
Target: 5'- aGAUCGCUUGCaGGUUaGACC-GCGAGUg -3' miRNA: 3'- -CUGGUGAGCG-CCAGaUUGGaCGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 4818 | 0.72 | 0.433805 |
Target: 5'- cGACUGCUCG-GGUCUggUuCUGCAgacGGCa -3' miRNA: 3'- -CUGGUGAGCgCCAGAuuG-GACGU---UCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 43094 | 0.76 | 0.251387 |
Target: 5'- aGCCGCUUGCGGcCUGGCCgGUucAGCa -3' miRNA: 3'- cUGGUGAGCGCCaGAUUGGaCGu-UCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 47001 | 0.76 | 0.232074 |
Target: 5'- aGACCGCU-GCGGUCUGaACCcuggUGUAGGCc -3' miRNA: 3'- -CUGGUGAgCGCCAGAU-UGG----ACGUUCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 47064 | 0.81 | 0.122209 |
Target: 5'- aGACCGCa-GCGGUCU-ACCUGCAcGCa -3' miRNA: 3'- -CUGGUGagCGCCAGAuUGGACGUuCG- -5' |
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7262 | 5' | -53.5 | NC_001900.1 | + | 2510 | 1.13 | 0.000644 |
Target: 5'- cGACCACUCGCGGUCUAACCUGCAAGCg -3' miRNA: 3'- -CUGGUGAGCGCCAGAUUGGACGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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