Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7265 | 3' | -59.1 | NC_001900.1 | + | 42013 | 0.66 | 0.458092 |
Target: 5'- cGCUCAaCGUcuccacGGUCAAGaACCGGGGUg -3' miRNA: 3'- -UGAGUaGCG------CCGGUUCgUGGUCCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 28050 | 0.66 | 0.458092 |
Target: 5'- cAC-CGUCacaGGCCGugaGGC-CCAGGGCGa -3' miRNA: 3'- -UGaGUAGcg-CCGGU---UCGuGGUCCCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 23220 | 0.66 | 0.458092 |
Target: 5'- aACUCGUCuGCcaucGCCGAGCugCAGGcucGCc -3' miRNA: 3'- -UGAGUAG-CGc---CGGUUCGugGUCC---CGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 3351 | 0.66 | 0.458092 |
Target: 5'- aGCggaaGUCGCGGCCGGugACCAG-GCAg -3' miRNA: 3'- -UGag--UAGCGCCGGUUcgUGGUCcCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 23500 | 0.66 | 0.455126 |
Target: 5'- cGCUCGUCGUcggcaccauccccGGUgAGGCugcaggccucuuCCGGGGCGg -3' miRNA: 3'- -UGAGUAGCG-------------CCGgUUCGu-----------GGUCCCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 16818 | 0.66 | 0.448246 |
Target: 5'- cGCgaaggCGUCGUcucaGGUUcAGCACCuGGGCAa -3' miRNA: 3'- -UGa----GUAGCG----CCGGuUCGUGGuCCCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 32076 | 0.66 | 0.428912 |
Target: 5'- uGCUCGUUGCGacaugcucaGCCuccguAGCugUAGGGUu -3' miRNA: 3'- -UGAGUAGCGC---------CGGu----UCGugGUCCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 14229 | 0.66 | 0.423208 |
Target: 5'- ---gGUCGCGGCCAGGCACgucgauguccgcaagCGGGuCAa -3' miRNA: 3'- ugagUAGCGCCGGUUCGUG---------------GUCCcGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 13939 | 0.66 | 0.419431 |
Target: 5'- aGCUCuUCGgGGUagaCGAGCACCGGcuGGUAc -3' miRNA: 3'- -UGAGuAGCgCCG---GUUCGUGGUC--CCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 20240 | 0.66 | 0.41849 |
Target: 5'- aGCUC-UCGCugagccacuaccuGGCCAAGUgggugaugaaccACCGGGGUc -3' miRNA: 3'- -UGAGuAGCG-------------CCGGUUCG------------UGGUCCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 10553 | 0.67 | 0.400857 |
Target: 5'- gGCcCAUCGCGGCCucuuccucGUcCCAGcGGCGc -3' miRNA: 3'- -UGaGUAGCGCCGGuu------CGuGGUC-CCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 43841 | 0.67 | 0.400857 |
Target: 5'- --gCGUCGggaaCGGCC-AGCGCgAGGGCu -3' miRNA: 3'- ugaGUAGC----GCCGGuUCGUGgUCCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 40396 | 0.67 | 0.400857 |
Target: 5'- cACUCGaCGCGGCCAaccacGGC-CgCAGcGGCu -3' miRNA: 3'- -UGAGUaGCGCCGGU-----UCGuG-GUC-CCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 3046 | 0.67 | 0.399942 |
Target: 5'- gUUgAUCGCGGUCAGcuucaucGCACCGauGGGCu -3' miRNA: 3'- uGAgUAGCGCCGGUU-------CGUGGU--CCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 7841 | 0.67 | 0.382815 |
Target: 5'- cAC-CGUCGCGGCCGucaGCCAGGa-- -3' miRNA: 3'- -UGaGUAGCGCCGGUucgUGGUCCcgu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 46516 | 0.67 | 0.382815 |
Target: 5'- uGCUCAcggUGCGGCCAugaggcgcagaGGUACCGaGGCc -3' miRNA: 3'- -UGAGUa--GCGCCGGU-----------UCGUGGUcCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 36058 | 0.68 | 0.340133 |
Target: 5'- gGCgaaCAUCGCgaucaugcuGGUCGAGCACCccGGGCu -3' miRNA: 3'- -UGa--GUAGCG---------CCGGUUCGUGGu-CCCGu -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 19262 | 0.68 | 0.332022 |
Target: 5'- aACUCggUGUcccaGGUagCGAGCACCGGGGUAg -3' miRNA: 3'- -UGAGuaGCG----CCG--GUUCGUGGUCCCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 6851 | 0.68 | 0.31934 |
Target: 5'- aGC-CAUCGUGGUuggccaggugcuCAAGCACCAcauccugccuccgacGGGCAg -3' miRNA: 3'- -UGaGUAGCGCCG------------GUUCGUGGU---------------CCCGU- -5' |
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7265 | 3' | -59.1 | NC_001900.1 | + | 153 | 0.7 | 0.239511 |
Target: 5'- gGCUCcUgGUGGCuCAcAGCGCCuGGGCGa -3' miRNA: 3'- -UGAGuAgCGCCG-GU-UCGUGGuCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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