miRNA: 3'- acgUGGUCCAcAUGuaGUgGCCCCGG- -5'
miRNA: 3'- aCGUGGUCCAcaUGUa-GUGGCCCc-GG- -5'
miRNA: 3'- aCGUGG-----------UCCACAUGUA--GUGGcC-CCGG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7267 | 5' | -57.4 | NC_001900.1 | + | 29828 | 0.69 | 0.395209 |
|
Target: 5'- --gGCCGGGUcUACggCGgUGGGGCCa -3' miRNA: 3'- acgUGGUCCAcAUGuaGUgGCCCCGG- -5' |
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| 7267 | 5' | -57.4 | NC_001900.1 | + | 19432 | 0.69 | 0.368641 |
|
Target: 5'- aGCGCCAGGU--ACAggUACCGGGuaCCg -3' miRNA: 3'- aCGUGGUCCAcaUGUa-GUGGCCCc-GG- -5' |
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| 7267 | 5' | -57.4 | NC_001900.1 | + | 3512 | 0.66 | 0.576694 |
|
Target: 5'- gGUGCCguugagguucaGGGUGUACGUgcCACCuGuGGCCc -3' miRNA: 3'- aCGUGG-----------UCCACAUGUA--GUGGcC-CCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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