Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7268 | 3' | -54.1 | NC_001900.1 | + | 40408 | 0.66 | 0.713759 |
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Target: 5'- cUCACCGAgaGCCCgugcagGAACa-CCAGCg- -3' miRNA: 3'- -AGUGGCUg-UGGGa-----CUUGaaGGUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 40498 | 0.77 | 0.180769 |
|
Target: 5'- gCACCGGCGCCCcgcUGGugUUCCugcacgGGCUCu -3' miRNA: 3'- aGUGGCUGUGGG---ACUugAAGG------UCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 41630 | 0.72 | 0.365936 |
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Target: 5'- gCACCGA--CCCUGGAUcUCCAGCa- -3' miRNA: 3'- aGUGGCUguGGGACUUGaAGGUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 43207 | 0.66 | 0.724674 |
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Target: 5'- -aGgCGGCACCC-GAGCg--CAGCUCg -3' miRNA: 3'- agUgGCUGUGGGaCUUGaagGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 44356 | 0.66 | 0.735493 |
|
Target: 5'- -gACCGGgaACCaaccggGAgacgACUUCCAGCUCg -3' miRNA: 3'- agUGGCUg-UGGga----CU----UGAAGGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 44628 | 0.68 | 0.624407 |
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Target: 5'- uUCACCGAggagACCCcGAACUUCgAGUg- -3' miRNA: 3'- -AGUGGCUg---UGGGaCUUGAAGgUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 47883 | 0.69 | 0.568417 |
|
Target: 5'- aCACCGuucguCGCaCCUGAGCUgucgaCGGUUCg -3' miRNA: 3'- aGUGGCu----GUG-GGACUUGAag---GUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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