Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7268 | 3' | -54.1 | NC_001900.1 | + | 47883 | 0.69 | 0.568417 |
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Target: 5'- aCACCGuucguCGCaCCUGAGCUgucgaCGGUUCg -3' miRNA: 3'- aGUGGCu----GUG-GGACUUGAag---GUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 4889 | 0.69 | 0.557346 |
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Target: 5'- ---aCGGCucgACUgaGAGCUUCCGGCUCg -3' miRNA: 3'- agugGCUG---UGGgaCUUGAAGGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 10998 | 0.7 | 0.50308 |
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Target: 5'- aUCAUCGGCGCUgUcaaGAACgacCCAGCUCc -3' miRNA: 3'- -AGUGGCUGUGGgA---CUUGaa-GGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 41630 | 0.72 | 0.365936 |
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Target: 5'- gCACCGA--CCCUGGAUcUCCAGCa- -3' miRNA: 3'- aGUGGCUguGGGACUUGaAGGUCGag -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 35111 | 0.73 | 0.357168 |
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Target: 5'- gUCGCaGACGaUgUGGACUUCCAGCUCc -3' miRNA: 3'- -AGUGgCUGUgGgACUUGAAGGUCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 40498 | 0.77 | 0.180769 |
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Target: 5'- gCACCGGCGCCCcgcUGGugUUCCugcacgGGCUCu -3' miRNA: 3'- aGUGGCUGUGGG---ACUugAAGG------UCGAG- -5' |
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| 7268 | 3' | -54.1 | NC_001900.1 | + | 3726 | 1.11 | 0.000759 |
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Target: 5'- gUCACCGACACCCUGAACUUCCAGCUCa -3' miRNA: 3'- -AGUGGCUGUGGGACUUGAAGGUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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