Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
727 | 5' | -44.7 | AC_000020.1 | + | 87 | 1.1 | 0.004658 |
Target: 5'- cCUUGCAACACCCAAAUUUAAGCCACAc -3' miRNA: 3'- -GAACGUUGUGGGUUUAAAUUCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 4359 | 0.7 | 0.896946 |
Target: 5'- aCUUGCcACACCCug---UGGGCC-CAu -3' miRNA: 3'- -GAACGuUGUGGGuuuaaAUUCGGuGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 4614 | 0.69 | 0.926134 |
Target: 5'- uUUGCAACcuguCCCAGAggcuuUUUAAGUCAUc -3' miRNA: 3'- gAACGUUGu---GGGUUU-----AAAUUCGGUGu -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 6206 | 0.68 | 0.963481 |
Target: 5'- uCUUGCAACACuCCAAug-UAAGUgGg- -3' miRNA: 3'- -GAACGUUGUG-GGUUuaaAUUCGgUgu -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 7259 | 0.68 | 0.949577 |
Target: 5'- aUUGCAuCACuCCAGAaaaaaGAGCCAUAg -3' miRNA: 3'- gAACGUuGUG-GGUUUaaa--UUCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 10003 | 0.71 | 0.862215 |
Target: 5'- gCUUGCGACACUUAAAguaUGAGUCGgGg -3' miRNA: 3'- -GAACGUUGUGGGUUUaa-AUUCGGUgU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 13589 | 0.72 | 0.83285 |
Target: 5'- --gGCAGgACCguAGUUaUAGGCCACAa -3' miRNA: 3'- gaaCGUUgUGGguUUAA-AUUCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 16922 | 0.66 | 0.980296 |
Target: 5'- --gGCAAC-CCCGucGAUgcu-GCCACAa -3' miRNA: 3'- gaaCGUUGuGGGU--UUAaauuCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 25376 | 0.69 | 0.926134 |
Target: 5'- cCUUGCcuccuACACCUAGAagUGGGCUugGg -3' miRNA: 3'- -GAACGu----UGUGGGUUUaaAUUCGGugU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 27370 | 0.67 | 0.977516 |
Target: 5'- ---aCAACGCCCuAGU---AGCCACAc -3' miRNA: 3'- gaacGUUGUGGGuUUAaauUCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 27922 | 0.67 | 0.967452 |
Target: 5'- --aGCcACGCCCA------AGCCACAc -3' miRNA: 3'- gaaCGuUGUGGGUuuaaauUCGGUGU- -5' |
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727 | 5' | -44.7 | AC_000020.1 | + | 31237 | 1.1 | 0.004658 |
Target: 5'- cCUUGCAACACCCAAAUUUAAGCCACAc -3' miRNA: 3'- -GAACGUUGUGGGUUUAAAUUCGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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