Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 7270 | 3' | -57.6 | NC_001900.1 | + | 14510 | 0.66 | 0.57757 |
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Target: 5'- cCGCcAUCGGCGGC---ACCGUCUCGu -3' miRNA: 3'- -GUGcUAGCUGCCGcacUGGCGGAGCu -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 21268 | 0.66 | 0.588262 |
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Target: 5'- gCGCGGUCGGUGGCGcGAUCGaCCUg-- -3' miRNA: 3'- -GUGCUAGCUGCCGCaCUGGC-GGAgcu -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 3126 | 0.66 | 0.609743 |
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Target: 5'- --gGGUCGGCGG-GUGcCCG-CUCGAu -3' miRNA: 3'- gugCUAGCUGCCgCACuGGCgGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 17086 | 0.66 | 0.588262 |
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Target: 5'- uCAUGGUCu-CGGC-UG-CCGCCUUGAu -3' miRNA: 3'- -GUGCUAGcuGCCGcACuGGCGGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 5227 | 0.66 | 0.588262 |
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Target: 5'- aGCGGUCGuuccaGGCGcGugCGCCgUCGu -3' miRNA: 3'- gUGCUAGCug---CCGCaCugGCGG-AGCu -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 43682 | 0.66 | 0.598988 |
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Target: 5'- gCAUGAgaCGGCGGCGguccugcggaUGcACCGCgUCGGa -3' miRNA: 3'- -GUGCUa-GCUGCCGC----------AC-UGGCGgAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 48754 | 0.66 | 0.598988 |
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Target: 5'- gACGAcagCGACaGCGUGACgacaGCC-CGAc -3' miRNA: 3'- gUGCUa--GCUGcCGCACUGg---CGGaGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 47358 | 0.66 | 0.598988 |
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Target: 5'- uCGCGAUgccuaCGGUGGCGUuGCCGCCUUu- -3' miRNA: 3'- -GUGCUA-----GCUGCCGCAcUGGCGGAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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