Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7270 | 3' | -57.6 | NC_001900.1 | + | 29663 | 0.67 | 0.535309 |
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Target: 5'- -cCGAUgGGcCGGCGgcugccggugGACCGCuCUCGGa -3' miRNA: 3'- guGCUAgCU-GCCGCa---------CUGGCG-GAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 7144 | 0.67 | 0.535309 |
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Target: 5'- uCACcGUCGugGGCGUagucGCgGaCCUCGAa -3' miRNA: 3'- -GUGcUAGCugCCGCAc---UGgC-GGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 17125 | 0.67 | 0.535309 |
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Target: 5'- -uCGAggaGACGGCGgucuUGGCCGCUUCc- -3' miRNA: 3'- guGCUag-CUGCCGC----ACUGGCGGAGcu -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 34840 | 0.67 | 0.524906 |
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Target: 5'- -uCGAggaCGGCGGUGcgcUGACCGgUCUCGAu -3' miRNA: 3'- guGCUa--GCUGCCGC---ACUGGC-GGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 29117 | 0.67 | 0.524906 |
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Target: 5'- gCACGAUCGAgggagacgagauCGGCGUcuacACCGCCgaugucccCGAa -3' miRNA: 3'- -GUGCUAGCU------------GCCGCAc---UGGCGGa-------GCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 33128 | 0.69 | 0.407572 |
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Target: 5'- gGCGGUagCGGCGGCgGUGGCCagcagcaccaGUCUCGGg -3' miRNA: 3'- gUGCUA--GCUGCCG-CACUGG----------CGGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 15203 | 0.7 | 0.355375 |
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Target: 5'- aGCGugccUCGGCuGGUGUGGCCGaCgCUCGAc -3' miRNA: 3'- gUGCu---AGCUG-CCGCACUGGC-G-GAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 3126 | 0.66 | 0.609743 |
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Target: 5'- --gGGUCGGCGG-GUGcCCG-CUCGAu -3' miRNA: 3'- gugCUAGCUGCCgCACuGGCgGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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