Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7270 | 3' | -57.6 | NC_001900.1 | + | 5970 | 0.69 | 0.397648 |
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Target: 5'- aACGG-CGACGGCauggGGCCGCCcgcuacgUCGAg -3' miRNA: 3'- gUGCUaGCUGCCGca--CUGGCGG-------AGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 22598 | 0.7 | 0.36374 |
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Target: 5'- --aGAUCGGCGGCgGUGAUuucaGCUUCGGc -3' miRNA: 3'- gugCUAGCUGCCG-CACUGg---CGGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 15203 | 0.7 | 0.355375 |
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Target: 5'- aGCGugccUCGGCuGGUGUGGCCGaCgCUCGAc -3' miRNA: 3'- gUGCu---AGCUG-CCGCACUGGC-G-GAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 11780 | 0.71 | 0.339054 |
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Target: 5'- aGCGAguUCaGCGGUGUGagcgucguucGCCGCCUUGAc -3' miRNA: 3'- gUGCU--AGcUGCCGCAC----------UGGCGGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 2369 | 0.71 | 0.308067 |
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Target: 5'- -uCGAUCuGGCuGGCGUGuuCCGCCUCa- -3' miRNA: 3'- guGCUAG-CUG-CCGCACu-GGCGGAGcu -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 5770 | 0.71 | 0.308067 |
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Target: 5'- -uUGAUcCGGC-GCGUGACCGUUUCGAg -3' miRNA: 3'- guGCUA-GCUGcCGCACUGGCGGAGCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 13774 | 0.73 | 0.239583 |
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Target: 5'- uCGCGGcgUCGACuuggucaGGUGUGACCGCCagGAg -3' miRNA: 3'- -GUGCU--AGCUG-------CCGCACUGGCGGagCU- -5' |
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| 7270 | 3' | -57.6 | NC_001900.1 | + | 4047 | 1.08 | 0.000758 |
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Target: 5'- uCACGAUCGACGGCGUGACCGCCUCGAu -3' miRNA: 3'- -GUGCUAGCUGCCGCACUGGCGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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