Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7270 | 5' | -55.3 | NC_001900.1 | + | 15610 | 0.7 | 0.436157 |
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Target: 5'- -cCCGGcgAGAGGCCGAacAGAUCCuCGu -3' miRNA: 3'- caGGCU--UCUCCGGCUguUCUAGG-GCu -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 22122 | 0.7 | 0.4265 |
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Target: 5'- --gCGAGGuGGGCCGAgAAGGacgcUCCCGAc -3' miRNA: 3'- cagGCUUC-UCCGGCUgUUCU----AGGGCU- -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 27899 | 0.71 | 0.389183 |
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Target: 5'- aGUCCGAucgcuGGGccgcuGCCGugGcGAUCCCGAg -3' miRNA: 3'- -CAGGCUu----CUC-----CGGCugUuCUAGGGCU- -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 23447 | 0.72 | 0.362636 |
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Target: 5'- cGUCCGuacGGAcccGGCCGACGAGAagacggUCaCCGAc -3' miRNA: 3'- -CAGGCu--UCU---CCGGCUGUUCU------AG-GGCU- -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 22894 | 0.73 | 0.321256 |
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Target: 5'- -cCCGGAGAGGUcauCGGgGAGAUCCCc- -3' miRNA: 3'- caGGCUUCUCCG---GCUgUUCUAGGGcu -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 46715 | 0.73 | 0.290769 |
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Target: 5'- cGUUCGggGucGGCCGACGAGAcaUCgCGGa -3' miRNA: 3'- -CAGGCuuCu-CCGGCUGUUCU--AGgGCU- -5' |
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| 7270 | 5' | -55.3 | NC_001900.1 | + | 4083 | 1.09 | 0.000889 |
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Target: 5'- aGUCCGAAGAGGCCGACAAGAUCCCGAa -3' miRNA: 3'- -CAGGCUUCUCCGGCUGUUCUAGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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