Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7271 | 3' | -60.7 | NC_001900.1 | + | 29583 | 0.66 | 0.392183 |
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Target: 5'- aGAGCGGUccaccGGCA--GCcGCCGGCCc-- -3' miRNA: 3'- -CUCGCCA-----UCGUgaCGaCGGCCGGcac -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 37372 | 0.66 | 0.37807 |
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Target: 5'- gGAGCaGGccaagacgagguuccUGGCcCcGCUGCgGGCCGUGu -3' miRNA: 3'- -CUCG-CC---------------AUCGuGaCGACGgCCGGCAC- -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 1250 | 0.66 | 0.366005 |
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Target: 5'- cGGCGGcAGCGgaGCUGgCGGCgGg- -3' miRNA: 3'- cUCGCCaUCGUgaCGACgGCCGgCac -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 30566 | 0.67 | 0.338631 |
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Target: 5'- -uGCGcUGGCACcGCUggugcccgacgugaGCCGGCCGUu -3' miRNA: 3'- cuCGCcAUCGUGaCGA--------------CGGCCGGCAc -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 40210 | 0.68 | 0.273749 |
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Target: 5'- cAGCGGUccugcuGGaCGCgGCUGCUGGCCa-- -3' miRNA: 3'- cUCGCCA------UC-GUGaCGACGGCCGGcac -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 43562 | 0.68 | 0.260317 |
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Target: 5'- gGAGCaGGcGGCGCaccgaGCUgGCCGGCCGa- -3' miRNA: 3'- -CUCG-CCaUCGUGa----CGA-CGGCCGGCac -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 30503 | 0.71 | 0.180874 |
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Target: 5'- cAGCGGUGccagcGCACUGCagauCCGGgCCGUGa -3' miRNA: 3'- cUCGCCAU-----CGUGACGac--GGCC-GGCAC- -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 24812 | 0.84 | 0.018664 |
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Target: 5'- cGGGCGGUGGCACgcucaucGCUGCCGGUgGUGc -3' miRNA: 3'- -CUCGCCAUCGUGa------CGACGGCCGgCAC- -5' |
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| 7271 | 3' | -60.7 | NC_001900.1 | + | 5283 | 1.07 | 0.000303 |
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Target: 5'- cGAGCGGUAGCACUGCUGCCGGCCGUGc -3' miRNA: 3'- -CUCGCCAUCGUGACGACGGCCGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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