Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7274 | 3' | -55.6 | NC_001900.1 | + | 16904 | 0.66 | 0.690726 |
Target: 5'- cCAUCGC-CGCGCcgcuugUCGGcuUGgugGCUGg -3' miRNA: 3'- aGUAGCGuGCGCG------AGCCuuGCa--CGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 25410 | 0.66 | 0.690726 |
Target: 5'- uUCAUCGcCAUGCGC--GGAGuCGUGUg- -3' miRNA: 3'- -AGUAGC-GUGCGCGagCCUU-GCACGac -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 1468 | 0.66 | 0.668773 |
Target: 5'- cUCuUCGCuAUGCGCUUGGcauccGACGgugagUGCUGg -3' miRNA: 3'- -AGuAGCG-UGCGCGAGCC-----UUGC-----ACGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 30934 | 0.66 | 0.657739 |
Target: 5'- ---gUGCAUgagGCGCUCG--ACGUGCUGa -3' miRNA: 3'- aguaGCGUG---CGCGAGCcuUGCACGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 13949 | 0.67 | 0.595795 |
Target: 5'- -gAUCGCACGCuuccaggucGCcaacaguccgguacgUCGGcacGACGUGCUGa -3' miRNA: 3'- agUAGCGUGCG---------CG---------------AGCC---UUGCACGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 21435 | 0.67 | 0.569449 |
Target: 5'- cCAUCGCACGCaGCgUCGGGACu----- -3' miRNA: 3'- aGUAGCGUGCG-CG-AGCCUUGcacgac -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 48985 | 0.67 | 0.569449 |
Target: 5'- cUCGagGCACGCGUgcCGuGAGCGUuuGCUGu -3' miRNA: 3'- -AGUagCGUGCGCGa-GC-CUUGCA--CGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 22158 | 0.68 | 0.558553 |
Target: 5'- gUCGUgcCGCugGC-CUCGGAAgGccgGCUGg -3' miRNA: 3'- -AGUA--GCGugCGcGAGCCUUgCa--CGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 46104 | 0.68 | 0.515639 |
Target: 5'- cCAUgCGC-CGCGCUCGG--CG-GCUGg -3' miRNA: 3'- aGUA-GCGuGCGCGAGCCuuGCaCGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 5152 | 0.69 | 0.484346 |
Target: 5'- aCggCGCACGCGCcUGGAACGacccGCUc -3' miRNA: 3'- aGuaGCGUGCGCGaGCCUUGCa---CGAc -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 17712 | 0.69 | 0.47107 |
Target: 5'- ---aCGCugGCGUcagcgccgugaugaUCGGAGCGaGCUGa -3' miRNA: 3'- aguaGCGugCGCG--------------AGCCUUGCaCGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 42068 | 0.7 | 0.4247 |
Target: 5'- gUCGUUGUACGgGC-CGGuGACGUGgUGg -3' miRNA: 3'- -AGUAGCGUGCgCGaGCC-UUGCACgAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 3535 | 0.71 | 0.368785 |
Target: 5'- cCAUCGaCGCgGCGCUCGGGGCcgguaacggccugGUGCa- -3' miRNA: 3'- aGUAGC-GUG-CGCGAGCCUUG-------------CACGac -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 9054 | 0.73 | 0.268038 |
Target: 5'- -uGUCGuUugGCGUUCaGGACGUGCUGc -3' miRNA: 3'- agUAGC-GugCGCGAGcCUUGCACGAC- -5' |
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7274 | 3' | -55.6 | NC_001900.1 | + | 6089 | 1.1 | 0.000734 |
Target: 5'- uUCAUCGCACGCGCUCGGAACGUGCUGg -3' miRNA: 3'- -AGUAGCGUGCGCGAGCCUUGCACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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