Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7276 | 3' | -59.2 | NC_001900.1 | + | 2341 | 0.66 | 0.479416 |
Target: 5'- cCAACUGCUcgUCGCUGaccucgaccUGGCCgucaCCCGAg -3' miRNA: 3'- -GUUGAUGG--GGCGAC---------GCCGGaa--GGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 43078 | 0.66 | 0.46945 |
Target: 5'- aUggUUGCCcaggaucagCCGCuUGCGGCCUggCCGGu -3' miRNA: 3'- -GuuGAUGG---------GGCG-ACGCCGGAagGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 46995 | 0.66 | 0.459591 |
Target: 5'- gCAGgUAgaCCGCUGCGGUCUgaacCCUGGu -3' miRNA: 3'- -GUUgAUggGGCGACGCCGGAa---GGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 9466 | 0.66 | 0.459591 |
Target: 5'- uCGACaucgagGCCCCGCUG-GGCUacaCCGAc -3' miRNA: 3'- -GUUGa-----UGGGGCGACgCCGGaagGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 38989 | 0.66 | 0.449843 |
Target: 5'- uCAACUGCCUgGcCUGCGGag--UCCGAg -3' miRNA: 3'- -GUUGAUGGGgC-GACGCCggaaGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 27907 | 0.67 | 0.430696 |
Target: 5'- -cGCUGggCCGCUGCcguGGCgaUCCCGAg -3' miRNA: 3'- guUGAUggGGCGACG---CCGgaAGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 19667 | 0.67 | 0.402895 |
Target: 5'- gAGCccGCCCUGCUcGCGGCCgacCuuGAu -3' miRNA: 3'- gUUGa-UGGGGCGA-CGCCGGaa-GggCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 43922 | 0.67 | 0.402895 |
Target: 5'- uCGGCccaGCCCuCGC-GCuGGCCgUUCCCGAc -3' miRNA: 3'- -GUUGa--UGGG-GCGaCG-CCGG-AAGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 37348 | 0.68 | 0.374524 |
Target: 5'- ----gGCCCCGCUGCGGgCCguguacgacggCCCu- -3' miRNA: 3'- guugaUGGGGCGACGCC-GGaa---------GGGcu -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 21286 | 0.68 | 0.350834 |
Target: 5'- uCGAcCUGcCCCCGCUggGCGGUCUgauggaugcggUCCUGAa -3' miRNA: 3'- -GUU-GAU-GGGGCGA--CGCCGGA-----------AGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 5153 | 0.68 | 0.342635 |
Target: 5'- uCGAC-GCCUCGUUGCGaGaCCUUCaCCGGg -3' miRNA: 3'- -GUUGaUGGGGCGACGC-C-GGAAG-GGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 8036 | 0.69 | 0.318875 |
Target: 5'- -uGCUGCCUCGaUGCGGCCa--CCGGc -3' miRNA: 3'- guUGAUGGGGCgACGCCGGaagGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 23146 | 0.69 | 0.318875 |
Target: 5'- aGGCUACCUgGCUcGCGGUg-UCCCGc -3' miRNA: 3'- gUUGAUGGGgCGA-CGCCGgaAGGGCu -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 33022 | 0.69 | 0.296374 |
Target: 5'- --cCUGCgCCUGCUGCGGag--CCCGAg -3' miRNA: 3'- guuGAUG-GGGCGACGCCggaaGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 805 | 0.7 | 0.26231 |
Target: 5'- gCGGCUGCgguagacguuccagaCCGCUGCGGCCcguggcaugUCCCGc -3' miRNA: 3'- -GUUGAUGg--------------GGCGACGCCGGa--------AGGGCu -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 11372 | 0.7 | 0.261649 |
Target: 5'- gAGCUGCagagcgcguuCCUG-UGCGGCCUUUCCGGg -3' miRNA: 3'- gUUGAUG----------GGGCgACGCCGGAAGGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 33321 | 0.72 | 0.181897 |
Target: 5'- gCAGCUACUCCGCgccGUGGCUggUgCCGAa -3' miRNA: 3'- -GUUGAUGGGGCGa--CGCCGGa-AgGGCU- -5' |
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7276 | 3' | -59.2 | NC_001900.1 | + | 6718 | 1.08 | 0.000424 |
Target: 5'- gCAACUACCCCGCUGCGGCCUUCCCGAu -3' miRNA: 3'- -GUUGAUGGGGCGACGCCGGAAGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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