Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7276 | 5' | -56.1 | NC_001900.1 | + | 31952 | 0.66 | 0.676879 |
Target: 5'- cGUCGAGAGGGaG-GcCGCUGAuGCgGAa -3' miRNA: 3'- cCAGCUCUUCC-CaCaGCGGCU-CGaCU- -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 6793 | 0.66 | 0.665993 |
Target: 5'- -aUCGGGAaggccgcagcGGGGUaGUUGCCGAuggGCUGc -3' miRNA: 3'- ccAGCUCU----------UCCCA-CAGCGGCU---CGACu -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 35134 | 0.68 | 0.546328 |
Target: 5'- uGGcCGAGgcGGGUcaGUUGUCGAGCc-- -3' miRNA: 3'- -CCaGCUCuuCCCA--CAGCGGCUCGacu -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 32814 | 0.68 | 0.525122 |
Target: 5'- aGUUGuGAGGGGgccaGUCGUCGuuGCUGGc -3' miRNA: 3'- cCAGCuCUUCCCa---CAGCGGCu-CGACU- -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 5520 | 0.69 | 0.473609 |
Target: 5'- cGUCGAccuccGGGGUGUUGCCGAGg--- -3' miRNA: 3'- cCAGCUcu---UCCCACAGCGGCUCgacu -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 12561 | 0.7 | 0.443945 |
Target: 5'- aGGUCGGGAAcGGGcUGUCgguGCCGuGCa-- -3' miRNA: 3'- -CCAGCUCUU-CCC-ACAG---CGGCuCGacu -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 26279 | 0.7 | 0.42476 |
Target: 5'- cGGUCGAGGAGuacaccaagaaGUGgaUCGCCGAGCg-- -3' miRNA: 3'- -CCAGCUCUUCc----------CAC--AGCGGCUCGacu -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 43135 | 0.71 | 0.38792 |
Target: 5'- -aUCGAGcugcgcucGGGUGcCGCCuGAGCUGAu -3' miRNA: 3'- ccAGCUCuu------CCCACaGCGG-CUCGACU- -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 16049 | 0.73 | 0.294317 |
Target: 5'- uGGUgGAGAcgcaacuGGGGgaagccgcgcccUCGCCGAGCUGAu -3' miRNA: 3'- -CCAgCUCU-------UCCCac----------AGCGGCUCGACU- -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 37422 | 0.77 | 0.163405 |
Target: 5'- cGUCGGGcuGGGUGUCGCCGA--UGAa -3' miRNA: 3'- cCAGCUCuuCCCACAGCGGCUcgACU- -5' |
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7276 | 5' | -56.1 | NC_001900.1 | + | 6754 | 1.11 | 0.00057 |
Target: 5'- cGGUCGAGAAGGGUGUCGCCGAGCUGAu -3' miRNA: 3'- -CCAGCUCUUCCCACAGCGGCUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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