Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7277 | 3' | -53 | NC_001900.1 | + | 27087 | 0.66 | 0.793457 |
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Target: 5'- cACGGUcgccaucgucgcGACGaGgAUGCCUCCGUCGAu -3' miRNA: 3'- cUGCCA------------CUGUaCaUGCGGAGGUAGUU- -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 12544 | 0.66 | 0.772862 |
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Target: 5'- -uCGGUGcCGUGcauCGCCgcgCCGUCGAa -3' miRNA: 3'- cuGCCACuGUACau-GCGGa--GGUAGUU- -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 30263 | 0.66 | 0.771814 |
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Target: 5'- cGCGGUGAacuuGUGUcccagcuccucgaACGCCUCCG-CGAc -3' miRNA: 3'- cUGCCACUg---UACA-------------UGCGGAGGUaGUU- -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 25747 | 0.66 | 0.762323 |
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Target: 5'- -cCGGUGACc-GUGcCGCCUCaCGUCGc -3' miRNA: 3'- cuGCCACUGuaCAU-GCGGAG-GUAGUu -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 1742 | 0.66 | 0.745166 |
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Target: 5'- cGGCGG-GACGUGgcccuugccgaaccACGCCUCC-UCGu -3' miRNA: 3'- -CUGCCaCUGUACa-------------UGCGGAGGuAGUu -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 42956 | 0.66 | 0.740825 |
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Target: 5'- -uUGGUGugAc---CGCCUCCGUCGAg -3' miRNA: 3'- cuGCCACugUacauGCGGAGGUAGUU- -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 11585 | 0.67 | 0.729893 |
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Target: 5'- uGCGGUgucgGACAUGUGuuuuCGCCUCCugggCAu -3' miRNA: 3'- cUGCCA----CUGUACAU----GCGGAGGua--GUu -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 39905 | 0.69 | 0.605421 |
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Target: 5'- aGCGGUGuCGUGU-CGgCUUCAUCAGc -3' miRNA: 3'- cUGCCACuGUACAuGCgGAGGUAGUU- -5' |
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| 7277 | 3' | -53 | NC_001900.1 | + | 7087 | 1.07 | 0.0018 |
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Target: 5'- cGACGGUGACAUGUACGCCUCCAUCAAa -3' miRNA: 3'- -CUGCCACUGUACAUGCGGAGGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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