Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7277 | 5' | -53.5 | NC_001900.1 | + | 20854 | 0.66 | 0.781066 |
Target: 5'- cAUCga-ACGggUGCG-GGUCGCgGUCc -3' miRNA: 3'- -UGGacgUGCuuAUGCuCCAGCGgUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 31496 | 0.66 | 0.760341 |
Target: 5'- --gUGCGCGAGauggugGCGGGGUucaCGCCGUa -3' miRNA: 3'- uggACGUGCUUa-----UGCUCCA---GCGGUAg -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 9129 | 0.66 | 0.749765 |
Target: 5'- gGCCcGCACGGGccGgGuGGUgGCCGUCc -3' miRNA: 3'- -UGGaCGUGCUUa-UgCuCCAgCGGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 31898 | 0.66 | 0.739064 |
Target: 5'- aGCUcGCcCGAGaccuCGGGGUCGCCAa- -3' miRNA: 3'- -UGGaCGuGCUUau--GCUCCAGCGGUag -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 41704 | 0.66 | 0.739064 |
Target: 5'- uACCUGCugGAGaucCaGGGUCGgugcuCCAUCg -3' miRNA: 3'- -UGGACGugCUUau-GcUCCAGC-----GGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 38979 | 0.66 | 0.739064 |
Target: 5'- gGCCUGCG-GAGUcCGAGGggaCGUgAUCu -3' miRNA: 3'- -UGGACGUgCUUAuGCUCCa--GCGgUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 43201 | 0.67 | 0.732587 |
Target: 5'- gACCUGUucgucccacagcuccGCGAGgugGAGGUCGUgGUCc -3' miRNA: 3'- -UGGACG---------------UGCUUaugCUCCAGCGgUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 9032 | 0.67 | 0.695246 |
Target: 5'- -gCUGC-CGAAgcACcGGGUUGCCGUCg -3' miRNA: 3'- ugGACGuGCUUa-UGcUCCAGCGGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 28308 | 0.67 | 0.6841 |
Target: 5'- -aCUGCACGAcgcuaccgACGAGGcCGUCcgCa -3' miRNA: 3'- ugGACGUGCUua------UGCUCCaGCGGuaG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 46292 | 0.67 | 0.6841 |
Target: 5'- gACCgcgacgGCAaGAAUACGaAGGUaucggcgaGCCAUCg -3' miRNA: 3'- -UGGa-----CGUgCUUAUGC-UCCAg-------CGGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 31429 | 0.68 | 0.616533 |
Target: 5'- gACCUG-AUGAAg--GAGGUCGCCGa- -3' miRNA: 3'- -UGGACgUGCUUaugCUCCAGCGGUag -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 36809 | 0.68 | 0.616533 |
Target: 5'- aACCaGCuCGGGcuugACGuGGUUGCCGUCa -3' miRNA: 3'- -UGGaCGuGCUUa---UGCuCCAGCGGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 25285 | 0.69 | 0.594011 |
Target: 5'- gAUCUGCagauuGCGGAUGCuGGGGUUGUCgAUCa -3' miRNA: 3'- -UGGACG-----UGCUUAUG-CUCCAGCGG-UAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 35985 | 0.69 | 0.582797 |
Target: 5'- gACCaGCAUGAucGCGAuGuUCGCCGUCg -3' miRNA: 3'- -UGGaCGUGCUuaUGCUcC-AGCGGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 38857 | 0.7 | 0.510255 |
Target: 5'- aGCCUGCACGGaagcccagccgccgaGUAUuugGAGaGUCGCgGUCu -3' miRNA: 3'- -UGGACGUGCU---------------UAUG---CUC-CAGCGgUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 34727 | 0.73 | 0.376956 |
Target: 5'- cACaCUGCGCGAugGCGuGGUCGaucuCCAUCg -3' miRNA: 3'- -UG-GACGUGCUuaUGCuCCAGC----GGUAG- -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 33262 | 0.74 | 0.325345 |
Target: 5'- gACCUGgGCGuugaucccgACGAGGUUGCCAa- -3' miRNA: 3'- -UGGACgUGCuua------UGCUCCAGCGGUag -5' |
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7277 | 5' | -53.5 | NC_001900.1 | + | 7121 | 1.1 | 0.000989 |
Target: 5'- gACCUGCACGAAUACGAGGUCGCCAUCg -3' miRNA: 3'- -UGGACGUGCUUAUGCUCCAGCGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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