Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7278 | 5' | -63.2 | NC_001900.1 | + | 26017 | 0.66 | 0.282064 |
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Target: 5'- cGCCCGGAGUacguuguaggcgUGCCgCCGUg-CCGUCg -3' miRNA: 3'- -UGGGCCUCGa-----------ACGG-GGCAgaGGCGGa -5' |
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| 7278 | 5' | -63.2 | NC_001900.1 | + | 13579 | 0.69 | 0.194674 |
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Target: 5'- aGCCUGGAGCUgguagguguacGCCCCGUCggucUCCGa-- -3' miRNA: 3'- -UGGGCCUCGAa----------CGGGGCAG----AGGCgga -5' |
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| 7278 | 5' | -63.2 | NC_001900.1 | + | 3025 | 0.69 | 0.194674 |
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Target: 5'- cGCaCCGauGGGCUUGCCCUGgugCUUgGCCg -3' miRNA: 3'- -UG-GGC--CUCGAACGGGGCa--GAGgCGGa -5' |
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| 7278 | 5' | -63.2 | NC_001900.1 | + | 23205 | 0.69 | 0.189716 |
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Target: 5'- cGCCCGG-GC-UGCgCCCGUCagggCCGaCCa -3' miRNA: 3'- -UGGGCCuCGaACG-GGGCAGa---GGC-GGa -5' |
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| 7278 | 5' | -63.2 | NC_001900.1 | + | 7395 | 1.06 | 0.000251 |
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Target: 5'- cACCCGGAGCUUGCCCCGUCUCCGCCUc -3' miRNA: 3'- -UGGGCCUCGAACGGGGCAGAGGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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