Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
728 | 3' | -57.7 | AC_000020.1 | + | 14737 | 0.66 | 0.461654 |
Target: 5'- cCCCGGCGUCccuaCCAgugGCGgggucGGCGCg -3' miRNA: 3'- -GGGCCGCGGccg-GGUauaUGU-----UCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 31106 | 0.66 | 0.441577 |
Target: 5'- aCCCggGGCGCCGGCCaaaaGUccGCGGaacuCGCc -3' miRNA: 3'- -GGG--CCGCGGCCGGg---UAuaUGUUc---GCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 218 | 0.66 | 0.441577 |
Target: 5'- aCCCggGGCGCCGGCCaaaaGUccGCGGaacuCGCc -3' miRNA: 3'- -GGG--CCGCGGCCGGg---UAuaUGUUc---GCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 16478 | 0.67 | 0.375442 |
Target: 5'- aCCCGaaGCGCaGGCCCcgcuggugGUACAaauacccaaaaAGCGCc -3' miRNA: 3'- -GGGC--CGCGgCCGGGua------UAUGU-----------UCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 15905 | 0.67 | 0.365675 |
Target: 5'- gUCGGCGCgGacggcGCCCAcgcggcaUAcgcUGCGGGCGCa -3' miRNA: 3'- gGGCCGCGgC-----CGGGU-------AU---AUGUUCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 14260 | 0.67 | 0.357815 |
Target: 5'- cCCCGcGgGCuCGGCCUugacgGCGgcGGCGCc -3' miRNA: 3'- -GGGC-CgCG-GCCGGGuaua-UGU--UCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 12656 | 0.68 | 0.332479 |
Target: 5'- uCCUGGCGCCGcugguaccCCCGcgGUACAaaGGgGCu -3' miRNA: 3'- -GGGCCGCGGCc-------GGGUa-UAUGU--UCgCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 27239 | 0.7 | 0.257706 |
Target: 5'- --aGGUGCCGGCCUugccGUGCAgAGCGg -3' miRNA: 3'- gggCCGCGGCCGGGua--UAUGU-UCGCg -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 9350 | 0.7 | 0.238125 |
Target: 5'- cCCUGGCGCaGGUUCA---GCAAGCGg -3' miRNA: 3'- -GGGCCGCGgCCGGGUauaUGUUCGCg -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 22952 | 0.71 | 0.225774 |
Target: 5'- gCCaGGCGCUuGCCCAUG---AGGCGCu -3' miRNA: 3'- gGG-CCGCGGcCGGGUAUaugUUCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 1889 | 0.72 | 0.185232 |
Target: 5'- aCCUGGCGguCUGGCuCCAUAUcuGCAuucugcuccaugccGGCGCa -3' miRNA: 3'- -GGGCCGC--GGCCG-GGUAUA--UGU--------------UCGCG- -5' |
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728 | 3' | -57.7 | AC_000020.1 | + | 2905 | 1.14 | 0.00013 |
Target: 5'- cCCCGGCGCCGGCCCAUAUACAAGCGCa -3' miRNA: 3'- -GGGCCGCGGCCGGGUAUAUGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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