Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7280 | 3' | -49.9 | NC_001900.1 | + | 6337 | 0.66 | 0.955906 |
Target: 5'- cGAUGUUCGcggcugcgcccgCGUcgaucccgccccagaUCAGGGCGAuGCCCa -3' miRNA: 3'- -CUGCAAGCa-----------GCA---------------AGUCUUGCUcUGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 12990 | 0.66 | 0.950616 |
Target: 5'- aGGCGUUCGUCGcucugaccaacguggUCAGcggcggcGGCGAGGgCUg -3' miRNA: 3'- -CUGCAAGCAGCa--------------AGUC-------UUGCUCUgGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 38314 | 0.66 | 0.940892 |
Target: 5'- gGAUGaUUCGUgCGUUCGGcAGCGucuuGGCCa -3' miRNA: 3'- -CUGC-AAGCA-GCAAGUC-UUGCu---CUGGg -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 9052 | 0.66 | 0.935597 |
Target: 5'- -uCGUUUGgCGUUCAGGACGuGcuGCCg -3' miRNA: 3'- cuGCAAGCaGCAAGUCUUGCuC--UGGg -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 46718 | 0.67 | 0.904733 |
Target: 5'- aGACGUUCGggg-UCGGccGACGAGACa- -3' miRNA: 3'- -CUGCAAGCagcaAGUC--UUGCUCUGgg -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 35999 | 0.67 | 0.904733 |
Target: 5'- cGAUGUUCGcCGUcgggUCAGAGu--GGCCCu -3' miRNA: 3'- -CUGCAAGCaGCA----AGUCUUgcuCUGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 9555 | 0.69 | 0.849683 |
Target: 5'- gGACGUaagCGUCGgugUAGcccAGCGGGGCCUc -3' miRNA: 3'- -CUGCAa--GCAGCaa-GUC---UUGCUCUGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 17708 | 0.69 | 0.831661 |
Target: 5'- uGGCGUcagCGcCGUgaugaUCGGAGCGAGcugaguGCCCa -3' miRNA: 3'- -CUGCAa--GCaGCA-----AGUCUUGCUC------UGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 10583 | 0.69 | 0.822311 |
Target: 5'- cGGCG-UCGaccaUCGUgcccagCAGGcCGAGGCCCa -3' miRNA: 3'- -CUGCaAGC----AGCAa-----GUCUuGCUCUGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 9083 | 0.7 | 0.81275 |
Target: 5'- -cUGUccUCG-CGUUCGGGGCGAGACa- -3' miRNA: 3'- cuGCA--AGCaGCAAGUCUUGCUCUGgg -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 24211 | 0.7 | 0.802987 |
Target: 5'- cGACGUgUUGUCGacCAGGugGAG-CCUa -3' miRNA: 3'- -CUGCA-AGCAGCaaGUCUugCUCuGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 41327 | 0.7 | 0.802987 |
Target: 5'- -cCGUUUGUCGUUuaagcCAGGGCGGuuCCCg -3' miRNA: 3'- cuGCAAGCAGCAA-----GUCUUGCUcuGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 34607 | 0.7 | 0.793035 |
Target: 5'- aGAUGUUCGUgGUcCAG--UGAGAUCCg -3' miRNA: 3'- -CUGCAAGCAgCAaGUCuuGCUCUGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 32441 | 0.71 | 0.762158 |
Target: 5'- gGACGUUCaGUCGUUCgcccaGGAggcguACGAGuACCUc -3' miRNA: 3'- -CUGCAAG-CAGCAAG-----UCU-----UGCUC-UGGG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 11843 | 0.71 | 0.73001 |
Target: 5'- cGCGUUCGUUGacaucuugCAGGGCGAGgACgCCg -3' miRNA: 3'- cUGCAAGCAGCaa------GUCUUGCUC-UG-GG- -5' |
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7280 | 3' | -49.9 | NC_001900.1 | + | 8022 | 1.14 | 0.001793 |
Target: 5'- cGACGUUCGUCGUUCAGAACGAGACCCa -3' miRNA: 3'- -CUGCAAGCAGCAAGUCUUGCUCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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