Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7281 | 3' | -58 | NC_001900.1 | + | 8787 | 0.66 | 0.53099 |
Target: 5'- --aCUGCAGCGUCCACGaaCUCgcggGGCAc -3' miRNA: 3'- cuaGACGUUGCGGGUGUagGGG----CCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 12003 | 0.66 | 0.53099 |
Target: 5'- cGAUCcuCAACGCCaugaaggGCGUUCUCGGCAu -3' miRNA: 3'- -CUAGacGUUGCGGg------UGUAGGGGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 1938 | 0.66 | 0.520399 |
Target: 5'- uGAUC-GCAuucggauuCGCCCGCAggccgaggccgUCaCCCGGCu -3' miRNA: 3'- -CUAGaCGUu-------GCGGGUGU-----------AG-GGGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 19174 | 0.66 | 0.489154 |
Target: 5'- cGUCUGUGgccaggaacACGCCCugGUCaCCGGUc -3' miRNA: 3'- cUAGACGU---------UGCGGGugUAGgGGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 29552 | 0.66 | 0.489154 |
Target: 5'- gGAUCUGguaguuCAGCGCCgagUACGUCCgagagcgguccaCCGGCAg -3' miRNA: 3'- -CUAGAC------GUUGCGG---GUGUAGG------------GGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 20785 | 0.66 | 0.478933 |
Target: 5'- gGAUCUGCuuCGCCgugucguagaACGUCCCgaacCGGCu -3' miRNA: 3'- -CUAGACGuuGCGGg---------UGUAGGG----GCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 40961 | 0.66 | 0.478933 |
Target: 5'- cGAUCUGCuGgGCCUGCAgaaUCCCgaacgGGCAu -3' miRNA: 3'- -CUAGACGuUgCGGGUGU---AGGGg----CCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 24048 | 0.67 | 0.458814 |
Target: 5'- gGAUCUGCAggAUGCCgACGaCgCCGGUu -3' miRNA: 3'- -CUAGACGU--UGCGGgUGUaGgGGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 13751 | 0.67 | 0.429507 |
Target: 5'- cGGUCUGCuacuGCGUCCACGa--CUGGCGc -3' miRNA: 3'- -CUAGACGu---UGCGGGUGUaggGGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 6188 | 0.67 | 0.423779 |
Target: 5'- aGAUCcGUgaAACGCuCUACuacgucggcacucucGUCCCCGGCAu -3' miRNA: 3'- -CUAGaCG--UUGCG-GGUG---------------UAGGGGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 30576 | 0.68 | 0.401331 |
Target: 5'- gGAUCUGCAguGCGCUgGCA-CCgCUGGUg -3' miRNA: 3'- -CUAGACGU--UGCGGgUGUaGG-GGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 18734 | 0.68 | 0.383212 |
Target: 5'- --cCUGCAGCGCCgCACGg--CCGGCc -3' miRNA: 3'- cuaGACGUUGCGG-GUGUaggGGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 25735 | 0.68 | 0.365645 |
Target: 5'- cGAUCUucucGcCGACGCgggCCGCG-CCCCGGCGc -3' miRNA: 3'- -CUAGA----C-GUUGCG---GGUGUaGGGGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 31417 | 0.69 | 0.340354 |
Target: 5'- cGAUCgGUAGCGCCU-CAUCaCgCGGCAg -3' miRNA: 3'- -CUAGaCGUUGCGGGuGUAG-GgGCCGU- -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 34461 | 0.75 | 0.135771 |
Target: 5'- --gCUGCugu-CCCACAUCCCCGGCu -3' miRNA: 3'- cuaGACGuugcGGGUGUAGGGGCCGu -5' |
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7281 | 3' | -58 | NC_001900.1 | + | 8151 | 1.08 | 0.000479 |
Target: 5'- cGAUCUGCAACGCCCACAUCCCCGGCAc -3' miRNA: 3'- -CUAGACGUUGCGGGUGUAGGGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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