Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7281 | 5' | -55.2 | NC_001900.1 | + | 16814 | 0.66 | 0.698127 |
Target: 5'- -aCGCCGcGAAGGCGU-CGUCucaGGUu -3' miRNA: 3'- caGCGGCuCUUCCGCAuGCAGc--UCAc -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 22758 | 0.66 | 0.686151 |
Target: 5'- cUCGCgGAGGAGG-GUGaucgcguUGUCGAGUc -3' miRNA: 3'- cAGCGgCUCUUCCgCAU-------GCAGCUCAc -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 44731 | 0.66 | 0.676304 |
Target: 5'- uGUCGCCGAGgcGGUcgagGUAUGg-GAGUc -3' miRNA: 3'- -CAGCGGCUCuuCCG----CAUGCagCUCAc -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 1142 | 0.67 | 0.654312 |
Target: 5'- --aGCCGGGGcAGGCG-GCGU-GGGUGg -3' miRNA: 3'- cagCGGCUCU-UCCGCaUGCAgCUCAC- -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 10424 | 0.67 | 0.632238 |
Target: 5'- cGUCaGCCuucgcGGCGUACGUCGGGg- -3' miRNA: 3'- -CAG-CGGcucuuCCGCAUGCAGCUCac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 26322 | 0.67 | 0.631133 |
Target: 5'- -cCGCCGgccgagcgcgagaAGAAGGC-UGCGgCGAGUGc -3' miRNA: 3'- caGCGGC-------------UCUUCCGcAUGCaGCUCAC- -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 25952 | 0.67 | 0.621195 |
Target: 5'- aGUgGCCGGGGAGGUGU---UCGAGc- -3' miRNA: 3'- -CAgCGGCUCUUCCGCAugcAGCUCac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 37073 | 0.67 | 0.621195 |
Target: 5'- -aCGCCGccacGguGGcCGUGCGUCGAGa- -3' miRNA: 3'- caGCGGCu---CuuCC-GCAUGCAGCUCac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 32428 | 0.68 | 0.599148 |
Target: 5'- uUCGCCcAGGAGGCGUA---CGAGUa -3' miRNA: 3'- cAGCGGcUCUUCCGCAUgcaGCUCAc -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 4012 | 0.68 | 0.588163 |
Target: 5'- uUCGCCGuGGAGGCGgguaccgGCGUCu---- -3' miRNA: 3'- cAGCGGCuCUUCCGCa------UGCAGcucac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 2221 | 0.68 | 0.55222 |
Target: 5'- gGUgGCUGGGAGGGCcuggagauccuugaGUAC-UCGGGUGa -3' miRNA: 3'- -CAgCGGCUCUUCCG--------------CAUGcAGCUCAC- -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 31975 | 0.69 | 0.544676 |
Target: 5'- -cCGCCGAGGAGaccgaGCGguucCGUCGAGa- -3' miRNA: 3'- caGCGGCUCUUC-----CGCau--GCAGCUCac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 21546 | 0.69 | 0.502282 |
Target: 5'- -aCGCCGAGAGGGUGaACGccuucaUCGGGcUGu -3' miRNA: 3'- caGCGGCUCUUCCGCaUGC------AGCUC-AC- -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 41833 | 0.7 | 0.481628 |
Target: 5'- cUCGCCGccGGucuGGGC-UugGUCGGGUGu -3' miRNA: 3'- cAGCGGC--UCu--UCCGcAugCAGCUCAC- -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 35261 | 0.72 | 0.376414 |
Target: 5'- -gCGCCucauGGAGGGCGgugGCGUCGGGg- -3' miRNA: 3'- caGCGGc---UCUUCCGCa--UGCAGCUCac -5' |
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7281 | 5' | -55.2 | NC_001900.1 | + | 8185 | 1.09 | 0.001003 |
Target: 5'- gGUCGCCGAGAAGGCGUACGUCGAGUGg -3' miRNA: 3'- -CAGCGGCUCUUCCGCAUGCAGCUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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