Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7282 | 3' | -55.6 | NC_001900.1 | + | 21273 | 0.66 | 0.698127 |
Target: 5'- cGCCaCCGAUgUucaccagggCCGAGAUCCCcUCguUGAu -3' miRNA: 3'- -UGG-GGCUAgA---------GGCUCUAGGGaAG--ACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 13662 | 0.66 | 0.665324 |
Target: 5'- gGCUCCGAUCaaguacccgCCGGGGUUUCUcCUGGc -3' miRNA: 3'- -UGGGGCUAGa--------GGCUCUAGGGAaGACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 3467 | 0.66 | 0.65872 |
Target: 5'- cACCCCGGccaUCgacuacaacgacgugUCCGAGAaCCCgcagggUCUGGc -3' miRNA: 3'- -UGGGGCU---AG---------------AGGCUCUaGGGa-----AGACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 33685 | 0.66 | 0.654312 |
Target: 5'- cGCCgCCGAUCUgCaGAGGUUCuCUgggCUGAu -3' miRNA: 3'- -UGG-GGCUAGAgG-CUCUAGG-GAa--GACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 2826 | 0.66 | 0.64328 |
Target: 5'- gUCCCGAUCaUCGAGAUCgUUUCg-- -3' miRNA: 3'- uGGGGCUAGaGGCUCUAGgGAAGacu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 29713 | 0.67 | 0.621195 |
Target: 5'- uUCgCGGUCUCgauCGAGAUCCCagUCUGc -3' miRNA: 3'- uGGgGCUAGAG---GCUCUAGGGa-AGACu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 14577 | 0.67 | 0.599148 |
Target: 5'- uGCUCCGAUcCUCCGAGAagaCCCUcgacUCg-- -3' miRNA: 3'- -UGGGGCUA-GAGGCUCUa--GGGA----AGacu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 18545 | 0.67 | 0.599148 |
Target: 5'- gACCCC----UCCGGGAUCCUcgCUGGu -3' miRNA: 3'- -UGGGGcuagAGGCUCUAGGGaaGACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 27315 | 0.67 | 0.577215 |
Target: 5'- aACCCCuGGUCagaagaggcUCUGAGAUCgaUUCUGAg -3' miRNA: 3'- -UGGGG-CUAG---------AGGCUCUAGggAAGACU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 47775 | 0.69 | 0.512754 |
Target: 5'- gUCCCGAcCUCCGAGAUCgCUgCaGAg -3' miRNA: 3'- uGGGGCUaGAGGCUCUAGgGAaGaCU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 16066 | 0.69 | 0.471456 |
Target: 5'- gGCCCgGAUC-CUGGGAUCCuCUUCc-- -3' miRNA: 3'- -UGGGgCUAGaGGCUCUAGG-GAAGacu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 913 | 0.7 | 0.441619 |
Target: 5'- gGCCCC---CUCCGGGAUCCCgUCg-- -3' miRNA: 3'- -UGGGGcuaGAGGCUCUAGGGaAGacu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 34896 | 0.7 | 0.422332 |
Target: 5'- gACCgaGGUCUCCGAGAUCCUgagCcGGu -3' miRNA: 3'- -UGGggCUAGAGGCUCUAGGGaa-GaCU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 26171 | 0.7 | 0.41288 |
Target: 5'- cACCCCGGUCgCCGAGAUgacccccgCCCUUg--- -3' miRNA: 3'- -UGGGGCUAGaGGCUCUA--------GGGAAgacu -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 37610 | 0.72 | 0.318021 |
Target: 5'- cGCCaaGAUCUCCGAgGGUCCgUUCgugGAg -3' miRNA: 3'- -UGGggCUAGAGGCU-CUAGGgAAGa--CU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 13558 | 0.73 | 0.287836 |
Target: 5'- cGCCCCGucgGUCUCCGAGAgguagCCCUcCgGGu -3' miRNA: 3'- -UGGGGC---UAGAGGCUCUa----GGGAaGaCU- -5' |
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7282 | 3' | -55.6 | NC_001900.1 | + | 8281 | 1.09 | 0.000889 |
Target: 5'- cACCCCGAUCUCCGAGAUCCCUUCUGAg -3' miRNA: 3'- -UGGGGCUAGAGGCUCUAGGGAAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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