Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7283 | 5' | -59.2 | NC_001900.1 | + | 4366 | 0.66 | 0.493328 |
Target: 5'- aGCcGCCGGuuccagguucGACUCCUGGCgUGUaugcCACCa -3' miRNA: 3'- -CGcCGGCUc---------CUGAGGACCG-ACA----GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 29916 | 0.66 | 0.488308 |
Target: 5'- cGCGGCC-AGGACgcgaagcuccugcCCUGGUaGUCgaucgagGCCa -3' miRNA: 3'- -CGCCGGcUCCUGa------------GGACCGaCAG-------UGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 39146 | 0.66 | 0.473395 |
Target: 5'- aCGGCCGGGGGCUUacgcaGGUaGUCgAUCg -3' miRNA: 3'- cGCCGGCUCCUGAGga---CCGaCAG-UGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 35910 | 0.66 | 0.463582 |
Target: 5'- cCGaGCCGAGGugUUCccgGaGCUGUucccaCACCa -3' miRNA: 3'- cGC-CGGCUCCugAGGa--C-CGACA-----GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 17295 | 0.66 | 0.463582 |
Target: 5'- uGCGGUCGAGGAUgUUCUGgaGCUGcuggaGCCc -3' miRNA: 3'- -CGCCGGCUCCUG-AGGAC--CGACag---UGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 29447 | 0.67 | 0.444281 |
Target: 5'- aUGGCCGAGGAgaugggUCCgGuGCUGaucggCACCg -3' miRNA: 3'- cGCCGGCUCCUg-----AGGaC-CGACa----GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 40882 | 0.67 | 0.444281 |
Target: 5'- aCGGUguGGGACgcCCUGGCUGacgagcugCACCg -3' miRNA: 3'- cGCCGgcUCCUGa-GGACCGACa-------GUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 2455 | 0.67 | 0.444281 |
Target: 5'- aCGGCCccGGcuGCgUCCgGGCUGcCACCg -3' miRNA: 3'- cGCCGGcuCC--UG-AGGaCCGACaGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 41475 | 0.67 | 0.434801 |
Target: 5'- gGgGGuuGGGGGCgcagGGC-GUCACCg -3' miRNA: 3'- -CgCCggCUCCUGaggaCCGaCAGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 6396 | 0.67 | 0.407082 |
Target: 5'- aGCGGCCGuGGcCggugcGGCUG-CGCCg -3' miRNA: 3'- -CGCCGGCuCCuGagga-CCGACaGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 18440 | 0.68 | 0.371898 |
Target: 5'- aCGGCCGAGGGCUUCggcaacGGCUucgaggaaggGUUccaGCCc -3' miRNA: 3'- cGCCGGCUCCUGAGGa-----CCGA----------CAG---UGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 3594 | 0.68 | 0.363431 |
Target: 5'- cCGcGUCGAuGGCgUCCUGGaUGUCACCg -3' miRNA: 3'- cGC-CGGCUcCUG-AGGACCgACAGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 35230 | 0.69 | 0.338842 |
Target: 5'- aGUGGuCCGAGGGCUUCUcGGUc--CACCc -3' miRNA: 3'- -CGCC-GGCUCCUGAGGA-CCGacaGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 37674 | 0.69 | 0.330919 |
Target: 5'- uCGGCCaAGGugUCCcacUGGCgagGuUCGCCc -3' miRNA: 3'- cGCCGGcUCCugAGG---ACCGa--C-AGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 45189 | 0.69 | 0.323133 |
Target: 5'- -aGGCUuggaugGAGGACgaUCCUucccaGGUUGUCACCc -3' miRNA: 3'- cgCCGG------CUCCUG--AGGA-----CCGACAGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 7181 | 0.69 | 0.315484 |
Target: 5'- gGUGGCCG-GGACUCCgUGGUa---GCCc -3' miRNA: 3'- -CGCCGGCuCCUGAGG-ACCGacagUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 20364 | 0.7 | 0.300599 |
Target: 5'- gGUGGUCaagGAGGACUCCggGGaCUGcUACCu -3' miRNA: 3'- -CGCCGG---CUCCUGAGGa-CC-GACaGUGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 35079 | 0.7 | 0.27247 |
Target: 5'- cGUGaUCGAGGACUCCcgcgagGGCUGggcaGCCg -3' miRNA: 3'- -CGCcGGCUCCUGAGGa-----CCGACag--UGG- -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 19256 | 0.71 | 0.246494 |
Target: 5'- cGCGaaGCa-GGGACcacggUCCUGGCUGUCGCg -3' miRNA: 3'- -CGC--CGgcUCCUG-----AGGACCGACAGUGg -5' |
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7283 | 5' | -59.2 | NC_001900.1 | + | 44513 | 0.71 | 0.233101 |
Target: 5'- cGUGGCgGGGGACUCCcGGCcaggagaucuggGUgACCc -3' miRNA: 3'- -CGCCGgCUCCUGAGGaCCGa-----------CAgUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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