Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7283 | 5' | -59.2 | NC_001900.1 | + | 35910 | 0.66 | 0.463582 |
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Target: 5'- cCGaGCCGAGGugUUCccgGaGCUGUucccaCACCa -3' miRNA: 3'- cGC-CGGCUCCugAGGa--C-CGACA-----GUGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 17295 | 0.66 | 0.463582 |
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Target: 5'- uGCGGUCGAGGAUgUUCUGgaGCUGcuggaGCCc -3' miRNA: 3'- -CGCCGGCUCCUG-AGGAC--CGACag---UGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 39146 | 0.66 | 0.473395 |
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Target: 5'- aCGGCCGGGGGCUUacgcaGGUaGUCgAUCg -3' miRNA: 3'- cGCCGGCUCCUGAGga---CCGaCAG-UGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 29916 | 0.66 | 0.488308 |
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Target: 5'- cGCGGCC-AGGACgcgaagcuccugcCCUGGUaGUCgaucgagGCCa -3' miRNA: 3'- -CGCCGGcUCCUGa------------GGACCGaCAG-------UGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 4366 | 0.66 | 0.493328 |
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Target: 5'- aGCcGCCGGuuccagguucGACUCCUGGCgUGUaugcCACCa -3' miRNA: 3'- -CGcCGGCUc---------CUGAGGACCG-ACA----GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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