miRNA: 3'- -CGCCGGCuCCUGAGG-ACCGacagUGG- -5'
miRNA: 3'- -CGCCGGCuCCuGagga-CCGACaGUGG- -5'
miRNA: 3'- -CGcCGGCUc---------CUGAGGACCG-ACA----GUGG- -5'
miRNA: 3'- cGC-CGGCUcCUG-AGGACCgACAGUGG- -5'
miRNA: 3'- cGCCGGcuCC--UG-AGGaCCGACaGUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7283 | 5' | -59.2 | NC_001900.1 | + | 7181 | 0.69 | 0.315484 |
|
Target: 5'- gGUGGCCG-GGACUCCgUGGUa---GCCc -3' miRNA: 3'- -CGCCGGCuCCUGAGG-ACCGacagUGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 6396 | 0.67 | 0.407082 |
|
Target: 5'- aGCGGCCGuGGcCggugcGGCUG-CGCCg -3' miRNA: 3'- -CGCCGGCuCCuGagga-CCGACaGUGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 4366 | 0.66 | 0.493328 |
|
Target: 5'- aGCcGCCGGuuccagguucGACUCCUGGCgUGUaugcCACCa -3' miRNA: 3'- -CGcCGGCUc---------CUGAGGACCG-ACA----GUGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 3594 | 0.68 | 0.363431 |
|
Target: 5'- cCGcGUCGAuGGCgUCCUGGaUGUCACCg -3' miRNA: 3'- cGC-CGGCUcCUG-AGGACCgACAGUGG- -5' |
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| 7283 | 5' | -59.2 | NC_001900.1 | + | 2455 | 0.67 | 0.444281 |
|
Target: 5'- aCGGCCccGGcuGCgUCCgGGCUGcCACCg -3' miRNA: 3'- cGCCGGcuCC--UG-AGGaCCGACaGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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