Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7284 | 3' | -51.7 | NC_001900.1 | + | 215 | 0.66 | 0.876618 |
Target: 5'- aGACU-CCUUcccuauuaAUGAGgGgcuGAAGGCCCc -3' miRNA: 3'- cCUGAuGGAG--------UACUCgUu--CUUCCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 40728 | 0.66 | 0.868502 |
Target: 5'- cGGACUGgga-GUGgcacGGCAAGAAGGCUg -3' miRNA: 3'- -CCUGAUggagUAC----UCGUUCUUCCGGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 13482 | 0.66 | 0.868502 |
Target: 5'- aGGGCUACCUCucgGAGacCGAcGGGGCg- -3' miRNA: 3'- -CCUGAUGGAGua-CUC--GUUcUUCCGgg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 12151 | 0.66 | 0.851491 |
Target: 5'- aGGGCUACCUg--GAGCccGAGcAGGCg- -3' miRNA: 3'- -CCUGAUGGAguaCUCG--UUCuUCCGgg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 12178 | 0.67 | 0.824157 |
Target: 5'- aGGACUACUUCGccGAGgcCGAGAAGaCCUc -3' miRNA: 3'- -CCUGAUGGAGUa-CUC--GUUCUUCcGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 2575 | 0.67 | 0.824157 |
Target: 5'- aGGGCguaGCC-CGacGGCGGGAacucaAGGCCCg -3' miRNA: 3'- -CCUGa--UGGaGUacUCGUUCU-----UCCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 47398 | 0.68 | 0.774446 |
Target: 5'- cGGuCUGCCcaGUGGGCAAGGGuucuccuGCCCg -3' miRNA: 3'- -CCuGAUGGagUACUCGUUCUUc------CGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 41792 | 0.68 | 0.76293 |
Target: 5'- cGACgugGCCUCAgagaaccUGAGCA---AGGCCg -3' miRNA: 3'- cCUGa--UGGAGU-------ACUCGUucuUCCGGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 41592 | 0.69 | 0.731781 |
Target: 5'- cGGACaacgucacgUACUUCGUgGAGCAGaucGAAGGCgCg -3' miRNA: 3'- -CCUG---------AUGGAGUA-CUCGUU---CUUCCGgG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 5164 | 0.69 | 0.720817 |
Target: 5'- gGGACUcccagucgacGCCUCGUu-GCGAGAccuucaccGGGCCa -3' miRNA: 3'- -CCUGA----------UGGAGUAcuCGUUCU--------UCCGGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 35718 | 0.69 | 0.68741 |
Target: 5'- uGGcGCUGgUUCcgGGGCAAGAAGGgCg -3' miRNA: 3'- -CC-UGAUgGAGuaCUCGUUCUUCCgGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 47342 | 0.7 | 0.619384 |
Target: 5'- aGGugUGCUaCGcGGGCAagcuggAGAAGGUCCg -3' miRNA: 3'- -CCugAUGGaGUaCUCGU------UCUUCCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 44266 | 0.71 | 0.574117 |
Target: 5'- cGACUACUUCAuggUGGGgAAGAAGGgCa -3' miRNA: 3'- cCUGAUGGAGU---ACUCgUUCUUCCgGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 11249 | 0.72 | 0.537354 |
Target: 5'- cGACUGCCUgGUGGuGCcugucuucgacgugGAGAAcuGGCCCg -3' miRNA: 3'- cCUGAUGGAgUACU-CG--------------UUCUU--CCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 10583 | 0.72 | 0.507893 |
Target: 5'- cGGCgucgACCaUCGUGcccAGCAGGccGAGGCCCa -3' miRNA: 3'- cCUGa---UGG-AGUAC---UCGUUC--UUCCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 15481 | 0.73 | 0.497154 |
Target: 5'- cGGACaccgGCCUUGUGGGCGuGguGGCCa -3' miRNA: 3'- -CCUGa---UGGAGUACUCGUuCuuCCGGg -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 15792 | 0.73 | 0.455286 |
Target: 5'- -cGCUGCUgaagC-UGGGCcAGAAGGCCCg -3' miRNA: 3'- ccUGAUGGa---GuACUCGuUCUUCCGGG- -5' |
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7284 | 3' | -51.7 | NC_001900.1 | + | 9107 | 1.16 | 0.000675 |
Target: 5'- aGGACUACCUCAUGAGCAAGAAGGCCCg -3' miRNA: 3'- -CCUGAUGGAGUACUCGUUCUUCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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