Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7284 | 5' | -59.3 | NC_001900.1 | + | 2436 | 0.66 | 0.514554 |
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Target: 5'- ----gGGUGGCUGUgUGAggucGACGGCCc -3' miRNA: 3'- gcccaCCACCGGCAgGCUa---CUGUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 1270 | 0.66 | 0.504389 |
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Target: 5'- gCGGGUuc-GGUgGUUCGAccGGCAGCCa -3' miRNA: 3'- -GCCCAccaCCGgCAGGCUa-CUGUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 9226 | 0.67 | 0.435955 |
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Target: 5'- cCGGGgagcgGGCUGUCaucgGACGGCCa -3' miRNA: 3'- -GCCCacca-CCGGCAGgcuaCUGUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 29314 | 0.67 | 0.435955 |
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Target: 5'- aGGGUGGcucgucGCCGUCgGAUGACcuUCg -3' miRNA: 3'- gCCCACCac----CGGCAGgCUACUGucGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 6694 | 0.67 | 0.408303 |
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Target: 5'- uCGGGcUGGcccGuGCCGUgaaCGGUGAaCAGCCa -3' miRNA: 3'- -GCCC-ACCa--C-CGGCAg--GCUACU-GUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 15558 | 0.67 | 0.408303 |
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Target: 5'- cCGGGcGGUGGa-GcCUGAcccaaacuUGACAGCCa -3' miRNA: 3'- -GCCCaCCACCggCaGGCU--------ACUGUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 10879 | 0.69 | 0.348234 |
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Target: 5'- cCGGGUGGUGGa-GUCUGcgcUGGgAGUCg -3' miRNA: 3'- -GCCCACCACCggCAGGCu--ACUgUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 2219 | 0.7 | 0.29047 |
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Target: 5'- gGGGUGGcugggagGGCCuGgagauccuugaguacUCgGGUGACGGCCa -3' miRNA: 3'- gCCCACCa------CCGG-C---------------AGgCUACUGUCGG- -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 33160 | 0.72 | 0.223967 |
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Target: 5'- uGGGUGGcggcagcaaGCCGaacUCCGGUGGCGGCg -3' miRNA: 3'- gCCCACCac-------CGGC---AGGCUACUGUCGg -5' |
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| 7284 | 5' | -59.3 | NC_001900.1 | + | 9141 | 1.1 | 0.000315 |
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Target: 5'- cCGGGUGGUGGCCGUCCGAUGACAGCCc -3' miRNA: 3'- -GCCCACCACCGGCAGGCUACUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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