Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7286 | 3' | -59.7 | NC_001900.1 | + | 13309 | 0.67 | 0.441236 |
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Target: 5'- cUCCuCGGUcgcaGGcUCCUGCUUCgGAGCgGg -3' miRNA: 3'- cAGG-GCCA----CC-AGGGCGAAGgCUUGgC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 31685 | 0.67 | 0.441236 |
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Target: 5'- -cUCCGGUcGG-CCCGaaccggCCGAACCGc -3' miRNA: 3'- caGGGCCA-CCaGGGCgaa---GGCUUGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 32241 | 0.67 | 0.431852 |
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Target: 5'- -gCCCGGUGGUugCCCGUga-CGAccAUCGg -3' miRNA: 3'- caGGGCCACCA--GGGCGaagGCU--UGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 8752 | 0.67 | 0.421665 |
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Target: 5'- cUCCCGGUacgacguGGUCgCGU-UCCGcGCCGa -3' miRNA: 3'- cAGGGCCA-------CCAGgGCGaAGGCuUGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 32598 | 0.67 | 0.395517 |
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Target: 5'- --gCCGGUcGGUCCUGa--CCGAACUGg -3' miRNA: 3'- cagGGCCA-CCAGGGCgaaGGCUUGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 10846 | 0.7 | 0.277946 |
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Target: 5'- aGUCCCGGcccaGGUCggcaGCUUCCGcauACCGa -3' miRNA: 3'- -CAGGGCCa---CCAGgg--CGAAGGCu--UGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 25020 | 0.7 | 0.277946 |
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Target: 5'- --gCCGGUGGUCCCGgUgcCCGuGGCCa -3' miRNA: 3'- cagGGCCACCAGGGCgAa-GGC-UUGGc -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 36261 | 0.71 | 0.233378 |
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Target: 5'- cGUCCCGGagaggGGUUCgGCUgCCGAGgCGa -3' miRNA: 3'- -CAGGGCCa----CCAGGgCGAaGGCUUgGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 11569 | 0.71 | 0.233378 |
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Target: 5'- -cCCCGGUGGUCacugacaagCCGCUg--GAACCGa -3' miRNA: 3'- caGGGCCACCAG---------GGCGAaggCUUGGC- -5' |
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| 7286 | 3' | -59.7 | NC_001900.1 | + | 9785 | 1.09 | 0.000398 |
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Target: 5'- gGUCCCGGUGGUCCCGCUUCCGAACCGg -3' miRNA: 3'- -CAGGGCCACCAGGGCGAAGGCUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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