Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7286 | 5' | -56 | NC_001900.1 | + | 43866 | 0.66 | 0.669095 |
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Target: 5'- -cUGGGCCgacugGUGCcacuucGGCAUgUCUGGGAu -3' miRNA: 3'- gaGCCUGGa----CAUG------CCGUGgAGACUCU- -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 17717 | 0.66 | 0.614024 |
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Target: 5'- uCUgGGGCCUGUGCuG-ACCguggucgCUGAGAc -3' miRNA: 3'- -GAgCCUGGACAUGcCgUGGa------GACUCU- -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 22576 | 0.67 | 0.559332 |
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Target: 5'- aUCGGAgCUG-ACGGCGCaUUC-GAGAu -3' miRNA: 3'- gAGCCUgGACaUGCCGUG-GAGaCUCU- -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 215 | 0.68 | 0.537808 |
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Target: 5'- -cCGGGCCUGUAUGGCccguaGCCgauUCcGGGGg -3' miRNA: 3'- gaGCCUGGACAUGCCG-----UGG---AGaCUCU- -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 34262 | 0.68 | 0.527152 |
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Target: 5'- aUCGG---UGUugGGCACCUCgGAGu -3' miRNA: 3'- gAGCCuggACAugCCGUGGAGaCUCu -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 4876 | 0.7 | 0.43553 |
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Target: 5'- aUCGGAaaCCgGUACGGCuCgaCUGAGAg -3' miRNA: 3'- gAGCCU--GGaCAUGCCGuGgaGACUCU- -5' |
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| 7286 | 5' | -56 | NC_001900.1 | + | 9820 | 1.08 | 0.00083 |
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Target: 5'- uCUCGGACCUGUACGGCACCUCUGAGAu -3' miRNA: 3'- -GAGCCUGGACAUGCCGUGGAGACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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