Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7288 | 3' | -53.6 | NC_001900.1 | + | 48636 | 0.66 | 0.781066 |
Target: 5'- gUGCCcgguaUGGGUGCUGUgaGCUGcACAAUCg -3' miRNA: 3'- -AUGG-----ACUCAUGGCGg-CGAC-UGUUAGg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 26354 | 0.66 | 0.770779 |
Target: 5'- gGCCcGaAGUACCGCUGCccGuCGAUCg -3' miRNA: 3'- aUGGaC-UCAUGGCGGCGa-CuGUUAGg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 30982 | 0.66 | 0.760341 |
Target: 5'- -uCCUGGGUGCUGCCcaucGAgGAUCUg -3' miRNA: 3'- auGGACUCAUGGCGGcga-CUgUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 46429 | 0.66 | 0.75929 |
Target: 5'- gGCCUcGGUACCucugcgccucaugGCCGCaccgUGAgCAGUCCc -3' miRNA: 3'- aUGGAcUCAUGG-------------CGGCG----ACU-GUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 25472 | 0.66 | 0.749765 |
Target: 5'- gGCCUGA--GCCGCCaGCgUGGC--UCCc -3' miRNA: 3'- aUGGACUcaUGGCGG-CG-ACUGuuAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 31836 | 0.66 | 0.739064 |
Target: 5'- gACCUGA---CCGCCGCgaACGGUCg -3' miRNA: 3'- aUGGACUcauGGCGGCGacUGUUAGg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 4104 | 0.66 | 0.739064 |
Target: 5'- -uCCcGAaccGCUGCCGCUGGCAGUUg -3' miRNA: 3'- auGGaCUca-UGGCGGCGACUGUUAGg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 1161 | 0.66 | 0.728248 |
Target: 5'- -uCCagGGGcACCGCCGCUaccGGCgGAUCCu -3' miRNA: 3'- auGGa-CUCaUGGCGGCGA---CUG-UUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 38759 | 0.66 | 0.728248 |
Target: 5'- -cCCUGGucaugagauGUACCgagGCUGcCUGGCGAUCCc -3' miRNA: 3'- auGGACU---------CAUGG---CGGC-GACUGUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 29020 | 0.67 | 0.717332 |
Target: 5'- gUACCUGGGU--CGgCGCUGAUGG-CCg -3' miRNA: 3'- -AUGGACUCAugGCgGCGACUGUUaGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 18059 | 0.67 | 0.706327 |
Target: 5'- aACCUGGcUGCgGCCguggugcagGCUGGCGcGUCCa -3' miRNA: 3'- aUGGACUcAUGgCGG---------CGACUGU-UAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 6564 | 0.67 | 0.672903 |
Target: 5'- gAgCUGGGggACCgcGCCGUUGACAGcggacUCCa -3' miRNA: 3'- aUgGACUCa-UGG--CGGCGACUGUU-----AGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 13169 | 0.68 | 0.661664 |
Target: 5'- cGCCUGA---UCGCgGCUGGCGGUUg -3' miRNA: 3'- aUGGACUcauGGCGgCGACUGUUAGg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 27908 | 0.68 | 0.639112 |
Target: 5'- --gCUGGGccGCUGCCG-UGGCGAUCCc -3' miRNA: 3'- augGACUCa-UGGCGGCgACUGUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 36357 | 0.69 | 0.582797 |
Target: 5'- gUACCUG-GUcgacaucCUGCCGCUGGCGAUg- -3' miRNA: 3'- -AUGGACuCAu------GGCGGCGACUGUUAgg -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 3275 | 0.69 | 0.560505 |
Target: 5'- cUGCCUGGucACCGgccgcgacuuCCGCUGGuCGAUCCc -3' miRNA: 3'- -AUGGACUcaUGGC----------GGCGACU-GUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 18863 | 0.7 | 0.538455 |
Target: 5'- gACCUGGGUAUCuCCGggGACGGUgCg -3' miRNA: 3'- aUGGACUCAUGGcGGCgaCUGUUAgG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 26171 | 0.72 | 0.433805 |
Target: 5'- -uCCUuGGUGCCGCCagcgaggucucGCUcGGCGAUCCa -3' miRNA: 3'- auGGAcUCAUGGCGG-----------CGA-CUGUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 39681 | 0.73 | 0.367981 |
Target: 5'- cACCUGugauGUAUCGCCGUggGAacaGAUCCa -3' miRNA: 3'- aUGGACu---CAUGGCGGCGa-CUg--UUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 43144 | 0.73 | 0.359154 |
Target: 5'- cGCUcGGGUGCCGCCugaGCUGAUgucccccagGAUCCa -3' miRNA: 3'- aUGGaCUCAUGGCGG---CGACUG---------UUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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