Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7288 | 3' | -53.6 | NC_001900.1 | + | 1161 | 0.66 | 0.728248 |
Target: 5'- -uCCagGGGcACCGCCGCUaccGGCgGAUCCu -3' miRNA: 3'- auGGa-CUCaUGGCGGCGA---CUG-UUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 38759 | 0.66 | 0.728248 |
Target: 5'- -cCCUGGucaugagauGUACCgagGCUGcCUGGCGAUCCc -3' miRNA: 3'- auGGACU---------CAUGG---CGGC-GACUGUUAGG- -5' |
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7288 | 3' | -53.6 | NC_001900.1 | + | 31836 | 0.66 | 0.739064 |
Target: 5'- gACCUGA---CCGCCGCgaACGGUCg -3' miRNA: 3'- aUGGACUcauGGCGGCGacUGUUAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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