miRNA: 3'- -CGGac--CACCACGGacAGaaGCUGCAC- -5'
miRNA: 3'- -CGGaC-CA---------CCACGGACaGAAGCUGCac -5'
miRNA: 3'- -CG-GACCA-CC-ACGGa-CAGAaGCUGCac -5'
miRNA: 3'- cGGACCa--------CCa-CGGACA-GAAGCUGCAC- -5'
miRNA: 3'- -CGGACCACCACGGACAGAAGCUGCAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7289 | 5' | -55.7 | NC_001900.1 | + | 12858 | 0.66 | 0.641367 |
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Target: 5'- aGCCgaccGUGGUGCCc-UCcaCGACGUGg -3' miRNA: 3'- -CGGac--CACCACGGacAGaaGCUGCAC- -5' |
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| 7289 | 5' | -55.7 | NC_001900.1 | + | 15114 | 0.67 | 0.597211 |
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Target: 5'- gGCCgGcGUaugugcagcGGUGCCUG-CUUCGGCGa- -3' miRNA: 3'- -CGGaC-CA---------CCACGGACaGAAGCUGCac -5' |
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| 7289 | 5' | -55.7 | NC_001900.1 | + | 8632 | 0.67 | 0.575275 |
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Target: 5'- gGCuCUGGUcGGcUGCCggGUCUcCGACGg- -3' miRNA: 3'- -CG-GACCA-CC-ACGGa-CAGAaGCUGCac -5' |
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| 7289 | 5' | -55.7 | NC_001900.1 | + | 12834 | 0.68 | 0.564374 |
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Target: 5'- aUCUGGggccagaucGGcgGUCUGUcCUUCGACGUGa -3' miRNA: 3'- cGGACCa--------CCa-CGGACA-GAAGCUGCAC- -5' |
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| 7289 | 5' | -55.7 | NC_001900.1 | + | 11253 | 1.11 | 0.000596 |
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Target: 5'- uGCCUGGUGGUGCCUGUCUUCGACGUGg -3' miRNA: 3'- -CGGACCACCACGGACAGAAGCUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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