Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
729 | 3' | -47.3 | AC_000020.1 | + | 9322 | 0.66 | 0.954488 |
Target: 5'- -gGCAGAGCGCGUaGUggcCGauuuGGCCCUu -3' miRNA: 3'- agUGUUUUGUGCA-CAa--GUgu--UCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 15554 | 0.68 | 0.891711 |
Target: 5'- aUCugGAAAgAUGgcaUUgGCGGGCCCCc -3' miRNA: 3'- -AGugUUUUgUGCac-AAgUGUUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 7506 | 0.68 | 0.874372 |
Target: 5'- aCACAgcgcAGACugGUGggaaaaaauaucuUUCACccccauugGGGCCCCg -3' miRNA: 3'- aGUGU----UUUGugCAC-------------AAGUG--------UUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 11397 | 0.68 | 0.866528 |
Target: 5'- aCACAAAGCcagUGUaGUUCuccuGGCCCCg -3' miRNA: 3'- aGUGUUUUGu--GCA-CAAGugu-UCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 15036 | 0.69 | 0.857531 |
Target: 5'- cUCGcCAGGACGCGc---CACGGGCUCCu -3' miRNA: 3'- -AGU-GUUUUGUGCacaaGUGUUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 15649 | 0.78 | 0.376855 |
Target: 5'- -gGCAGGAUGC-UGUUgGCAGGCCCCc -3' miRNA: 3'- agUGUUUUGUGcACAAgUGUUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 3296 | 1.14 | 0.001587 |
Target: 5'- gUCACAAAACACGUGUUCACAAGCCCCu -3' miRNA: 3'- -AGUGUUUUGUGCACAAGUGUUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 16471 | 0.74 | 0.562999 |
Target: 5'- aCAUAAAACcCGaag-CGCAGGCCCCg -3' miRNA: 3'- aGUGUUUUGuGCacaaGUGUUCGGGG- -5' |
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729 | 3' | -47.3 | AC_000020.1 | + | 19545 | 0.68 | 0.883626 |
Target: 5'- -gACGugGCACGcGUcCAUcAGCCCCa -3' miRNA: 3'- agUGUuuUGUGCaCAaGUGuUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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