Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 729 | 5' | -48.8 | AC_000020.1 | + | 6698 | 0.66 | 0.882846 |
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Target: 5'- aCCA-GGCUGUGAAUGaguuuUACAuGCUugGc -3' miRNA: 3'- -GGUaCCGACAUUUAU-----GUGU-CGGugCa -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 7460 | 0.68 | 0.786684 |
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Target: 5'- aCCAgucUGcGCUGUGu----GCGGCCACGUg -3' miRNA: 3'- -GGU---AC-CGACAUuuaugUGUCGGUGCA- -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 19605 | 0.69 | 0.742087 |
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Target: 5'- cCCGguggGGCUGaugg-ACGCGuGCCACGUc -3' miRNA: 3'- -GGUa---CCGACauuuaUGUGU-CGGUGCA- -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 13333 | 0.69 | 0.742087 |
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Target: 5'- gCCu--GCUGUGGGUaaaguccaccGCACAGCCugGa -3' miRNA: 3'- -GGuacCGACAUUUA----------UGUGUCGGugCa -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 10980 | 0.7 | 0.706006 |
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Target: 5'- gUCGUcGGCUGUAGGcugacucUGCAUGGCCGCc- -3' miRNA: 3'- -GGUA-CCGACAUUU-------AUGUGUCGGUGca -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 30187 | 0.7 | 0.695356 |
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Target: 5'- gCUGUGGCUGgAAAgccugcGCACAGCC-CGa -3' miRNA: 3'- -GGUACCGACaUUUa-----UGUGUCGGuGCa -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 3200 | 0.8 | 0.206112 |
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Target: 5'- aCGUGGCUGUGuAUuUACAGCCAUGg -3' miRNA: 3'- gGUACCGACAUuUAuGUGUCGGUGCa -5' |
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| 729 | 5' | -48.8 | AC_000020.1 | + | 3257 | 1.11 | 0.001689 |
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Target: 5'- uCCAUGGCUGUAAAUACACAGCCACGUa -3' miRNA: 3'- -GGUACCGACAUUUAUGUGUCGGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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