Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7290 | 3' | -55.5 | NC_001900.1 | + | 8432 | 0.66 | 0.677609 |
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Target: 5'- aACUCGAUCaCCGAGUCacgGCGcAAGu- -3' miRNA: 3'- cUGAGCUAGcGGCUCAGg--CGC-UUCuu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 4068 | 0.66 | 0.677609 |
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Target: 5'- -cCUCGAUCaagGUCGAGUCCGaaGAGGc -3' miRNA: 3'- cuGAGCUAG---CGGCUCAGGCgcUUCUu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 44402 | 0.66 | 0.655452 |
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Target: 5'- aGCUCGAUCGCUGGcguaccGUCCGgGGucacccAGAu -3' miRNA: 3'- cUGAGCUAGCGGCU------CAGGCgCU------UCUu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 12179 | 0.66 | 0.655452 |
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Target: 5'- gGACUacuUCGCCGAGgCCGaGAAGAc -3' miRNA: 3'- -CUGAgcuAGCGGCUCaGGCgCUUCUu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 16432 | 0.66 | 0.654341 |
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Target: 5'- -gUUCGuUCGCCGcgaugguAGUggCCGCGAAGAAg -3' miRNA: 3'- cuGAGCuAGCGGC-------UCA--GGCGCUUCUU- -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 26401 | 0.67 | 0.622077 |
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Target: 5'- cACUCGAccgcugCGCUGGGUCCGCa----- -3' miRNA: 3'- cUGAGCUa-----GCGGCUCAGGCGcuucuu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 9846 | 0.67 | 0.610956 |
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Target: 5'- gGACcacCGGgaccgCGCCGAGcCCGUGggGGu -3' miRNA: 3'- -CUGa--GCUa----GCGGCUCaGGCGCuuCUu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 9368 | 0.67 | 0.588781 |
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Target: 5'- cGACUCGAUCGCaGAGcgacaggCUGuCGAGGGu -3' miRNA: 3'- -CUGAGCUAGCGgCUCa------GGC-GCUUCUu -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 10445 | 0.71 | 0.366631 |
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Target: 5'- uACUCcAUCGCCGAG--CGCGAGGAGa -3' miRNA: 3'- cUGAGcUAGCGGCUCagGCGCUUCUU- -5' |
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| 7290 | 3' | -55.5 | NC_001900.1 | + | 11876 | 1.06 | 0.001213 |
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Target: 5'- cGACUCGAUCGCCGAGUCCGCGAAGAAg -3' miRNA: 3'- -CUGAGCUAGCGGCUCAGGCGCUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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