Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7290 | 5' | -49.3 | NC_001900.1 | + | 34540 | 0.66 | 0.931579 |
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Target: 5'- gAGCgcgGGUUGaGCGGg-UCGACAGGAGc -3' miRNA: 3'- -UUGa--CUAGC-CGCCaaAGCUGUUCUUc -5' |
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| 7290 | 5' | -49.3 | NC_001900.1 | + | 24590 | 0.66 | 0.92561 |
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Target: 5'- gAGCUGG-CGGUGGUg--GACAAGGc- -3' miRNA: 3'- -UUGACUaGCCGCCAaagCUGUUCUuc -5' |
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| 7290 | 5' | -49.3 | NC_001900.1 | + | 12844 | 0.69 | 0.840201 |
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Target: 5'- ---aGAUCGGCGGUcuguccUUCGACGuGAc- -3' miRNA: 3'- uugaCUAGCCGCCA------AAGCUGUuCUuc -5' |
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| 7290 | 5' | -49.3 | NC_001900.1 | + | 37180 | 0.72 | 0.657053 |
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Target: 5'- gAACUGGUCGGCGuacUCGACcAGAGc -3' miRNA: 3'- -UUGACUAGCCGCcaaAGCUGuUCUUc -5' |
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| 7290 | 5' | -49.3 | NC_001900.1 | + | 43813 | 0.81 | 0.226799 |
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Target: 5'- --aUGGUCGGCGGUggaaCGGCAAGGAGu -3' miRNA: 3'- uugACUAGCCGCCAaa--GCUGUUCUUC- -5' |
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| 7290 | 5' | -49.3 | NC_001900.1 | + | 11910 | 1.06 | 0.005047 |
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Target: 5'- gAACUGAUCGGCGGUUUCGACAAGAAGc -3' miRNA: 3'- -UUGACUAGCCGCCAAAGCUGUUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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