Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7291 | 5' | -50.4 | NC_001900.1 | + | 16878 | 0.66 | 0.934491 |
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Target: 5'- aGCGUggaUCGGCGUCGGGauc-GUCGc -3' miRNA: 3'- cCGCAag-AGCCGUAGUUCacuaCAGC- -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 13696 | 0.66 | 0.922833 |
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Target: 5'- uGGCGcUCaugUCGGCAUCAc--GAUGuUCGg -3' miRNA: 3'- -CCGCaAG---AGCCGUAGUucaCUAC-AGC- -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 24239 | 0.67 | 0.903129 |
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Target: 5'- cGGCGggagccgacaucUUCUgGGCAUCcgacGUGuUGUCGa -3' miRNA: 3'- -CCGC------------AAGAgCCGUAGuu--CACuACAGC- -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 12723 | 0.68 | 0.856055 |
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Target: 5'- cGcCGUUCaggaUCGGC-UC-GGUGAUGUCGu -3' miRNA: 3'- cC-GCAAG----AGCCGuAGuUCACUACAGC- -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 11945 | 0.68 | 0.856055 |
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Target: 5'- cGCGgaCUCGGCgAUCGAGUcGgcGUCc -3' miRNA: 3'- cCGCaaGAGCCG-UAGUUCA-CuaCAGc -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 26094 | 0.68 | 0.838273 |
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Target: 5'- gGGCGggggucaUCUCGGCGaCcGG-GGUGUCGc -3' miRNA: 3'- -CCGCa------AGAGCCGUaGuUCaCUACAGC- -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 34300 | 0.7 | 0.737212 |
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Target: 5'- cGGUGggCUCGG-AUCAGGUcGAUGUa- -3' miRNA: 3'- -CCGCaaGAGCCgUAGUUCA-CUACAgc -5' |
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| 7291 | 5' | -50.4 | NC_001900.1 | + | 12022 | 1.12 | 0.001816 |
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Target: 5'- gGGCGUUCUCGGCAUCAAGUGAUGUCGa -3' miRNA: 3'- -CCGCAAGAGCCGUAGUUCACUACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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