Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7293 | 5' | -59.7 | NC_001900.1 | + | 33033 | 0.66 | 0.463208 |
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Target: 5'- uGCGGAGcCCGagaCUGgugcUGCUGGCCaCCGCc -3' miRNA: 3'- -CGCCUCuGGCa--GGC----ACGGUUGG-GGCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 34463 | 0.67 | 0.433818 |
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Target: 5'- uGUGGAGagaucacGCUGgagCCGUGggAGCCCUGCa -3' miRNA: 3'- -CGCCUC-------UGGCa--GGCACggUUGGGGCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 38247 | 0.66 | 0.463208 |
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Target: 5'- -gGGuuGCCGcCCGUGCCAccggAUCCaGCa -3' miRNA: 3'- cgCCucUGGCaGGCACGGU----UGGGgCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 40639 | 0.66 | 0.462244 |
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Target: 5'- uGCGGAGgacgccaGCCuucuugCCGUGCCAcucccAgUCCGCa -3' miRNA: 3'- -CGCCUC-------UGGca----GGCACGGU-----UgGGGCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 41948 | 0.66 | 0.463208 |
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Target: 5'- cGUGGAGACguugagcgaGUCCGgGCCGAagCCGUc -3' miRNA: 3'- -CGCCUCUGg--------CAGGCaCGGUUggGGCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 43227 | 0.69 | 0.316602 |
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Target: 5'- gGUGGAGGucgUgGUCCGgGCCAGCCCaGUc -3' miRNA: 3'- -CGCCUCU---GgCAGGCaCGGUUGGGgCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 44702 | 0.66 | 0.492614 |
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Target: 5'- uGCGGcagccAGGCCGcuggCCGcUGCCAcACCuuGUc -3' miRNA: 3'- -CGCC-----UCUGGCa---GGC-ACGGU-UGGggCG- -5' |
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| 7293 | 5' | -59.7 | NC_001900.1 | + | 46623 | 0.68 | 0.360801 |
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Target: 5'- cGUGGuAGGuCCaGUCCGcgaugucucgucgGCCGACCCCGa -3' miRNA: 3'- -CGCC-UCU-GG-CAGGCa------------CGGUUGGGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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