Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7294 | 3' | -55.4 | NC_001900.1 | + | 10425 | 0.66 | 0.716313 |
Target: 5'- gCGuCAGCCU--UCgCGGCgUACGUCGg -3' miRNA: 3'- -GCuGUCGGGcaAGgGCUGgAUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 4149 | 0.66 | 0.705619 |
Target: 5'- uGuCGGCCucuuCGgaCUCGACCUugAUCGa -3' miRNA: 3'- gCuGUCGG----GCaaGGGCUGGAugUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 43301 | 0.66 | 0.705619 |
Target: 5'- gGGCuGGCCCGgaCCaCGACCUccACcUCGc -3' miRNA: 3'- gCUG-UCGGGCaaGG-GCUGGA--UGuAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 12724 | 0.66 | 0.694854 |
Target: 5'- aGGCcgcgAGCCCGUUCCguucgGGCCggauCGUCGc -3' miRNA: 3'- gCUG----UCGGGCAAGGg----CUGGau--GUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 19335 | 0.66 | 0.694854 |
Target: 5'- gCGACAGCcaggaCCGUggUCCCuGCUUcGCGUCGu -3' miRNA: 3'- -GCUGUCG-----GGCA--AGGGcUGGA-UGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 9445 | 0.66 | 0.673154 |
Target: 5'- uCGACAGCCUGUcgcUCUgCGAUCga-GUCGa -3' miRNA: 3'- -GCUGUCGGGCA---AGG-GCUGGaugUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 48201 | 0.67 | 0.662241 |
Target: 5'- -uGCGGCCC-UUCCCGGuCUUGCGUg- -3' miRNA: 3'- gcUGUCGGGcAAGGGCU-GGAUGUAgc -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 42346 | 0.67 | 0.662241 |
Target: 5'- uCGACuccucgauGCuCUGUUCCCGgacuGCCcGCGUCGa -3' miRNA: 3'- -GCUGu-------CG-GGCAAGGGC----UGGaUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 6820 | 0.67 | 0.618413 |
Target: 5'- gCGACA-CCC-UUCUCGACCgacggccACAUCGg -3' miRNA: 3'- -GCUGUcGGGcAAGGGCUGGa------UGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 36505 | 0.67 | 0.618413 |
Target: 5'- -uGCGGCCCGgugaggCCgUGAUCUACGUCc -3' miRNA: 3'- gcUGUCGGGCaa----GG-GCUGGAUGUAGc -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 9744 | 0.68 | 0.607462 |
Target: 5'- uGGCAGCCUGgaUCUCGuugcccUCUGCGUCGu -3' miRNA: 3'- gCUGUCGGGCa-AGGGCu-----GGAUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 8548 | 0.68 | 0.596531 |
Target: 5'- uGACAGCCaggaGUcCUCGGCC-GCGUUGa -3' miRNA: 3'- gCUGUCGGg---CAaGGGCUGGaUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 3334 | 0.68 | 0.57477 |
Target: 5'- -aGCAGCCCacccCCUGGCCUGCGggCGa -3' miRNA: 3'- gcUGUCGGGcaa-GGGCUGGAUGUa-GC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 35968 | 0.68 | 0.553194 |
Target: 5'- -cGCAGCCCGgggugCUCGACCaGCAUg- -3' miRNA: 3'- gcUGUCGGGCaa---GGGCUGGaUGUAgc -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 30073 | 0.69 | 0.510844 |
Target: 5'- aGACGcuGCCCGcgUCCGACUggacggUGCGUCGg -3' miRNA: 3'- gCUGU--CGGGCaaGGGCUGG------AUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 27170 | 0.7 | 0.479987 |
Target: 5'- gCGugGGCCaCGUUgCCCGcgACCgACAUCa -3' miRNA: 3'- -GCugUCGG-GCAA-GGGC--UGGaUGUAGc -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 19043 | 0.71 | 0.411835 |
Target: 5'- cCGACAGCgCGUUCuuGGauuccucgcgguCCUugAUCGa -3' miRNA: 3'- -GCUGUCGgGCAAGggCU------------GGAugUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 13980 | 0.71 | 0.411835 |
Target: 5'- uCGACGGCUgggucuucuUGUUCCCGuCC-GCGUCGa -3' miRNA: 3'- -GCUGUCGG---------GCAAGGGCuGGaUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 43036 | 0.71 | 0.411835 |
Target: 5'- gGACAGCCgGggagcgUCCCGG--UACAUCGg -3' miRNA: 3'- gCUGUCGGgCa-----AGGGCUggAUGUAGC- -5' |
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7294 | 3' | -55.4 | NC_001900.1 | + | 16756 | 0.73 | 0.333594 |
Target: 5'- gGGCAGCCuCGgUCuuGAgCUGCAUCa -3' miRNA: 3'- gCUGUCGG-GCaAGggCUgGAUGUAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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