Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7294 | 5' | -52.4 | NC_001900.1 | + | 40619 | 0.66 | 0.823285 |
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Target: 5'- aGCCcucaCCAUCUCGAUguUGCggagGACGCc -3' miRNA: 3'- -UGGuuccGGUAGAGCUA--ACGa---CUGUG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 16656 | 0.66 | 0.813617 |
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Target: 5'- gGCCAAGGCCGaCUUcaagcgGCUGAUgauGCa -3' miRNA: 3'- -UGGUUCCGGUaGAGcuaa--CGACUG---UG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 27541 | 0.66 | 0.803741 |
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Target: 5'- gGCuCAAGGCCGUgcggCUCGGccccagGCUGAUccGCg -3' miRNA: 3'- -UG-GUUCCGGUA----GAGCUaa----CGACUG--UG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 24795 | 0.66 | 0.803741 |
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Target: 5'- cACCAcaGCCAccuUCUCGGgcGgUGGCACg -3' miRNA: 3'- -UGGUucCGGU---AGAGCUaaCgACUGUG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 24481 | 0.67 | 0.740814 |
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Target: 5'- gACCAGguguGGCCAgcgUUCGAcccggUGCUGACu- -3' miRNA: 3'- -UGGUU----CCGGUa--GAGCUa----ACGACUGug -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 2954 | 0.69 | 0.627748 |
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Target: 5'- cACCAGGGCaagccCAUCggugCGAUgaaGCUGAcCGCg -3' miRNA: 3'- -UGGUUCCG-----GUAGa---GCUAa--CGACU-GUG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 23714 | 0.7 | 0.536901 |
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Target: 5'- aGCCGGGaCCAUCUucacguUGGUcGCUGGCACg -3' miRNA: 3'- -UGGUUCcGGUAGA------GCUAaCGACUGUG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 18559 | 0.74 | 0.355257 |
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Target: 5'- cGCCAGGGCCuuggccuUCUCGAUgacggGCUGGaACc -3' miRNA: 3'- -UGGUUCCGGu------AGAGCUAa----CGACUgUG- -5' |
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| 7294 | 5' | -52.4 | NC_001900.1 | + | 12527 | 1.09 | 0.001495 |
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Target: 5'- cACCAAGGCCAUCUCGAUUGCUGACACc -3' miRNA: 3'- -UGGUUCCGGUAGAGCUAACGACUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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