Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
7295 | 5' | -54.6 | NC_001900.1 | + | 37930 | 0.71 | 0.430106 |
Target: 5'- aUCGCCCuGCUGUUC-UCGGG-CuACCu -3' miRNA: 3'- cAGCGGG-CGACAAGuAGCUCaGcUGG- -5' |
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7295 | 5' | -54.6 | NC_001900.1 | + | 3726 | 0.71 | 0.420543 |
Target: 5'- aGUCGUUCGUggccuUGUUgAUCGuGUUGACCa -3' miRNA: 3'- -CAGCGGGCG-----ACAAgUAGCuCAGCUGG- -5' |
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7295 | 5' | -54.6 | NC_001900.1 | + | 14155 | 0.73 | 0.324369 |
Target: 5'- aUCGCCCGCacUGgcg--CGGGUCGACUa -3' miRNA: 3'- cAGCGGGCG--ACaaguaGCUCAGCUGG- -5' |
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7295 | 5' | -54.6 | NC_001900.1 | + | 12690 | 1.11 | 0.000781 |
Target: 5'- gGUCGCCCGCUGUUCAUCGAGUCGACCu -3' miRNA: 3'- -CAGCGGGCGACAAGUAGCUCAGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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