Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7296 | 5' | -52.2 | NC_001900.1 | + | 37104 | 0.66 | 0.855193 |
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Target: 5'- cGGCUGAcacCGCGUacaaCGCgaUGCAGCGg -3' miRNA: 3'- -CCGACUcauGCGCAag--GUG--ACGUUGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 32882 | 0.66 | 0.855193 |
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Target: 5'- uGCUGGGguacUGCGCGgggCACUGCucguuGCGa -3' miRNA: 3'- cCGACUC----AUGCGCaagGUGACGu----UGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 47046 | 0.67 | 0.809336 |
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Target: 5'- cGCUGaAGUcucggaugacACGCGcccaCGCUGCGACGu -3' miRNA: 3'- cCGAC-UCA----------UGCGCaag-GUGACGUUGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 804 | 0.67 | 0.789547 |
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Target: 5'- cGGCUGcGGUAgaCGUUCCagaccGCUGCGGCc -3' miRNA: 3'- -CCGAC-UCAUgcGCAAGG-----UGACGUUGc -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 8805 | 0.67 | 0.779388 |
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Target: 5'- cGGgaGA--ACGCGgaaUgCACUGCAGCGu -3' miRNA: 3'- -CCgaCUcaUGCGCa--AgGUGACGUUGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 18125 | 0.69 | 0.704457 |
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Target: 5'- cGGCUG-GUugGCG-UCCuggcagACUGCGuCGg -3' miRNA: 3'- -CCGACuCAugCGCaAGG------UGACGUuGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 27771 | 0.69 | 0.670979 |
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Target: 5'- cGCUGAGgcaaACGCacugcUCUACUGCGACc -3' miRNA: 3'- cCGACUCa---UGCGca---AGGUGACGUUGc -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 6452 | 0.71 | 0.566301 |
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Target: 5'- aGGCUGAGgucgaGCGCgucaagcaggcucuGgagUCCGCUGuCAACGg -3' miRNA: 3'- -CCGACUCa----UGCG--------------Ca--AGGUGAC-GUUGC- -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 11371 | 0.8 | 0.170693 |
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Target: 5'- aGCUGcAGaGCGCGUUCCugUGCGGCc -3' miRNA: 3'- cCGAC-UCaUGCGCAAGGugACGUUGc -5' |
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| 7296 | 5' | -52.2 | NC_001900.1 | + | 12958 | 0.99 | 0.009759 |
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Target: 5'- aGGCUGAGUACGCGUU-CACUGCAACGa -3' miRNA: 3'- -CCGACUCAUGCGCAAgGUGACGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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